Carregant...
Fitxers
Tipus de document
ArticleVersió
Versió acceptadaData de publicació
Tots els drets reservats
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/43402
Protein interaction studies point to new functions for Escherichia coli glyceraldehyde-3-phosphate dehydrogenase
Títol de la revista
Director/Tutor
ISSN de la revista
Títol del volum
Recurs relacionat
Resum
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is considered a multifunctional protein with defined functions in numerous mammalian cellular processes. GAPDH functional diversity depends on various factors such as covalent modifications, subcellular localization, oligomeric state and intracellular concentration of substrates or ligands, as well as protein-protein interactions. In bacteria, alternative GAPDH functions have been associated with its extracellular location in pathogens or probiotics. In this study, new intracellular functions of E. coli GAPDH were investigated following a proteomic approach aimed at identifying interacting partners using in vivo formaldehyde cross-linking followed by mass spectrometry. The identified proteins were involved in metabolic processes, protein synthesis and folding or DNA repair. Some interacting proteins were also identified in immunopurification experiments in the absence of cross-linking. Pull-down experiments and overlay immunoblotting were performed to further characterize the interaction with phosphoglycolate phosphatase (Gph). This enzyme is involved in the metabolism of 2-phosphoglycolate formed in the DNA repair of 3"-phosphoglycolate ends generated by bleomycin damage. We show that interaction between Gph and GAPDH increases in cells challenged with bleomycin, suggesting involvement of GAPDH in cellular processes linked to DNA repair mechanisms.
Matèries
Matèries (anglès)
Citació
Citació
FERREIRA, Elaine, GIMÉNEZ CLAUDIO, Rosa, AGUILERA GIL, Maria laura, GUZMÁN LÓPEZ, Karla, AGUILAR PIERA, Juan, BADÍA PALACÍN, Josefa, BALDOMÀ LLAVINÉS, Laura. Protein interaction studies point to new functions for Escherichia coli glyceraldehyde-3-phosphate dehydrogenase. _Research in Microbiology_. 2013. Vol. 164, núm. 145-154. [consulta: 14 de gener de 2026]. ISSN: 0923-2508. [Disponible a: https://hdl.handle.net/2445/43402]