Carregant...
Tipus de document
Treball de fi de grauData de publicació
Llicència de publicació
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/194500
Characterisation of the noise affecting magnetic tweezers’ measures
Títol de la revista
Autors
Director/Tutor
ISSN de la revista
Títol del volum
Recurs relacionat
Resum
Magnetic tweezers are used to study and manipulate biomolecules by using a microscope and a CCD camera to track the position of micron-sized magnetic beads inside a microfluidic chamber. In these experiments we can attach the molecule of interest to a bead, and adjust the position of a pair of magnets placed above the chamber to apply different forces to the tethered molecule and track the molecule extension in real time. The resolution of these measurements are mainly affected by three sources of noise: the tracking noise, the Brownian motion noise and the low-frequency noise. Here we characterised these types of noise. We found that the tracking noise increases with the frequency at which the camera track as expected for a Poissonian uncorrelated noise. For the Brownian case, the noise increased with the length of the tethered molecule and
decreases with the force applied. The dependencies found could be reproduced with a simple description
of an inverted overdamped pendulum and using standard polymer models for the molecule elasticity. The low-frequency noise was present in all the experiments and could be corrected by substracting the average position of fixed beads inside the chamber
Descripció
Treballs Finals de Grau de Física, Facultat de Física, Universitat de Barcelona, Curs: 2022-2023, Tutora: Maria Mañosas
Matèries
Matèries (anglès)
Citació
Col·leccions
Citació
SÁEZ ROS, Víctor. Characterisation of the noise affecting magnetic tweezers’ measures. [consulta: 9 de abril de 2026]. [Disponible a: https://hdl.handle.net/2445/194500]