Heterosis and reciprocal effects for physiological and morphological traits of popcorn plants under different water conditions

Abstract

In spite of the benefits of heterosis in maize breeding, little is known about the physiological mechanisms of this phenomenon and its genetic control under different water regimes. This study aimed to understand the heterosis effects on plant growth, the photosynthetic and transpiration traits, and the root traits of four inbred popcorn lines and their hybrids, including their reciprocal combinations. Plants were grown in lysimeters, inside a rain shelter, under two water conditions (water stress - WS; well-watered - WW) until anthesis. Plant growth traits included shoot biomass, plant height, and leaf area. Photosynthetic traits comprised leaf pigment and total nitrogen content, chlorophyll fluorescence, gas exchange, water use efficiency and stomatal index and density, along with the stable carbon (δ13C) and nitrogen (δ15N) isotope compositions of the last developed leaf. Root weight density and specific root length were also recorded. Greater heterosis effects were observed for traits related to plant growth and root weight density, and specifically under WS. Traits related to root weight density in deeper soil layers benefited markedly from heterosis, but there were no advantages in terms of stomatal conductance and water status in general. Apparently, only δ13C supported a better water status under WS, and was observed in the hybrids in particular. Non-additive gene effects were predominant in controlling of most of the growth and root traits studied, supporting the conclusion that the heterosis effect is especially favorable under water-limiting conditions. Moreover, the choice of the female parent is essential for traits related to gas exchange when breeding for better resilience to drought.