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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/156637
RNA-Seq transcriptomic profiling of primary murine microglia treated with LPS or LPS+IFNγ
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Microglia, the main resident immune cells in the CNS, are thought to participate in the pathogenesis of various neurological disorders. LPS and LPS + IFNγ are stimuli that are widely used to activate microglia. However, the transcriptomic profiles of microglia treated with LPS and LPS + IFNγ have not been properly compared. Here, we treated murine primary microglial cultures with LPS or LPS + IFNγ for 6 hours and then performed RNA-Sequencing. Gene expression patterns induced by the treatments were obtained by WGCNA and 11 different expression profiles were found, showing differential responses to LPS and LPS + IFNγ in many genes. Interestingly, a subset of genes involved in Parkinson's, Alzheimer's and Huntington's disease were downregulated by both treatments. By DESeq analysis we found differentially upregulated and downregulated genes that confirmed LPS and LPS + IFNγ as inducers of microglial pro-inflammatory responses, but also highlighted their involvement in specific cell functions. In response to LPS, microglia tended to be more proliferative, pro-inflammatory and phagocytic; whereas LPS + IFNγ inhibited genes were involved in pain, cell division and, unexpectedly, production of some inflammatory mediators. In summary, this study provides a detailed description of the transcriptome of LPS- and LPS + IFNγ treated primary microglial cultures. It may be useful to determine whether these in vitro phenotypes resemble microglia in in vivo pathological conditions.
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PULIDO SALGADO, Marta, VIDAL TABOADA, José manuel, GARCÍA-DÍAZ BARRIGA, Gerardo, SOLÀ I SUBIRANA, Carme, SAURA MARTÍ, Josep. RNA-Seq transcriptomic profiling of primary murine microglia treated with LPS or LPS+IFNγ. _Scientific Reports_. 2018. Vol. 8, núm. 16906. [consulta: 25 de febrer de 2026]. ISSN: 2045-2322. [Disponible a: https://hdl.handle.net/2445/156637]