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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/151389
Identification of antihypertensive peptides in nutraceuticals by capillary electrophoresis-mass spectrometry
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We present capillary electrophoresis-mass spectrometry (CE-MS) in combination with advanced chemometric tools for the analysis of bioactive compounds in food, in particular for the identification of antihypertensive peptides in a nutraceutical derived from a bovine milk protein hydrolysate. Different extracts of the nutraceutical were analyzed by CE-MS, and the electropherograms were processed using a novel data analysis workflow that included regions of interest (ROIs) compression and multivariate curve resolution alternating least squares (MCR-ALS). MCR-ALS permitted the description of the nutraceutical extract as ten characteristic components with their electrophoretic profiles and mass spectra. Twenty-two compounds were tentatively identified as antihypertensive bovine casein fragments through a mass search in a database of bioactive peptides. The identity of 17 antihypertensive peptides was reliably confirmed by capillary electrophoresis-tandem mass spectrometry. The proposed analytical approach demonstrated the potential to obtain a characteristic and activity-related fingerprint for quality control and authentication of the antihypertensive nutraceutical.
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BENAVENTE MORENO, Fernando J. (Julián), et al. Identification of antihypertensive peptides in nutraceuticals by capillary electrophoresis-mass spectrometry. Journal of Chromatography A. 2018. Vol. 1579, num. 129-137. ISSN 0021-9673. [consulted: 23 of May of 2026]. Available at: https://hdl.handle.net/2445/151389