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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/151262
Variable-temperature size exclusion chromatography for the study of the structural changes in G-quadruplex
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The conformational equilibria of a guanine-rich sequence found at the promoter region of the human c-kit oncogene are studied by means of circular dichroism spectroscopy (CD) and variable-temperature Size Exclusion Chromatography (SEC). It is shown that the wild sequence ckit21 exists as a mixture of monomeric and multimeric G-quadruplex. Appropriate mutation of several bases in the wild sequence produces the shift from parallel to antiparallel G-quadruplex, as well as the disappearance of multimeric species. The shift from the antiparallel to the parallel conformation induced by temperature is reflected in both CD and SEC profiles.
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BENABOU ZDAOU, Sanae, ERITJA I CASADELLÀ, Ramon and GARGALLO GÓMEZ, Raimundo. Variable-temperature size exclusion chromatography for the study of the structural changes in G-quadruplex. ISRN Biochemistry. 2013. Vol. 2013, num. 1-7. ISSN 2090-7729. [consulted: 6 of June of 2026]. Available at: https://hdl.handle.net/2445/151262