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Title: Challenges and future prospects on 3D in-vitro modeling of the neuromuscular circuit
Author: Badiola Mateos, Maider
Hervera Abad, Arnau
Río Fernández, José Antonio del
Samitier i Martí, Josep
Keywords: Malalties neuromusculars
Aparell locomotor
Neuromuscular diseases
Musculoskeletal system
Issue Date: 11-Dec-2018
Publisher: Frontiers Media
Abstract: Movement of skeletal-muscle fibers is generated by the coordinated action of several cells taking part within the locomotion circuit (motoneurons, sensory-neurons, Schwann cells, astrocytes, microglia, and muscle-cells). Failure s in any part of this circuit could impede or hinder coordinated muscle movement and cause a neu romuscular disease (NMD) or determine its severity. Studying fragments of the circuit cannot provide a comprehensive and complete view of the pathological process. We trace the historic developments of studies focused on in-vitro modeling of the spinal-locomotion circuit and how bioengineered innovative technologies show advantages for an accurate mimicking of hysiological conditions of spinal-locomotion circuit. New developments on compartmentalized microfluidic culture systems (cμFCS), the use of human induced pluripotent stem cells (hiPSCs) and 3D cell-cultures are analyzed. We finally address limitations of current study models and three main challenges on neuromuscular studies: (i) mimic the whole spinal-locomotion circuit including all cell-types involved and the evaluation of independent and interdependent roles of each one; (ii) mimic the neurodegenerative response of mature neurons in-vitro as it occurs in-vivo ; and (iii) develop, tune, implement, and combine cμFCS, hiPSC, and 3D-culture technologies to ultimately create patient-specific complete, translational, and reliable NMD in-vitro model. Overcoming these challenges would significantly facilitate understanding the events taking place in NMDs and accelerate the process of finding new therapies.
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It is part of: Frontiers in Bioengineering and Biotechnology, 2018, vol. 6, num. 194
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ISSN: 2296-4185
Appears in Collections:Articles publicats en revistes (Electrònica)

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