Llibres / Capítols de llibre (Institut de Nanociència i Nanotecnologia (IN2UB))
URI permanent per a aquesta col·leccióhttps://hdl.handle.net/2445/104466
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Part del llibrePolysiloxane Side-Chain Azobenzene-Containing Liquid Single Crystal Elastomers for Photo-Active Artificial(IntechOpen, 2012-09-12) Garcia Amorós, Jaume; Velasco Castrillo, DoloresLiquid crystals (LCs) are unique materials with amazing properties and uses. Since their discovery in 1889 by F. Reinitzer1, they have experienced an explosive growth because of their successful application in a wide variety of areas such as information displays2, cosmetics and health care3, thermography4-5, artificial muscle-like actuators6-7 and enantioselective synthesis8, among others. Thus, liquid crystals play an important role in modern technology and they are present in the most common devices used in our daily live. Research into this field is growing day by day and new promising applications for such materials are discovered and developed continuously...- Objecte de conferènciaStrategies against β-amyloid protein as therapeutics in Alzheimer's disease(Research Signpost, 2017) Folch, Jaume; Ettcheto Arriola, Miren; Busquets Figueras, Oriol; Sánchez-López, E. (Elena); Castro-Torres, Rubén Darío; Beas Zárate, Carlos; Pallàs i Llibería, Mercè, 1964-; Olloquequi, Jordi; Jara, Daniela; García López, María Luisa; Auladell i Costa, M. Carme; Camins Espuny, Antoni
- Part del llibreThe lipid-protein interplay in efflux plump(Research Signpost, 2016) Borrell Hernández, Jordi; Montero Barrientos, Ma. Teresa; Vázquez González, Martha Leticia; Domènech Cabrera, ÒscarIn this work lactose permease (LacY) of Escherichia coli has been taken as model efflux pump to investigate the interactions between the protein and the main lipid components (POPE and POPG) of the inner membrane. Two main approaches have been followed: (i) measuring the fluorescence energy transfer between a single tryptophan mutant of the protein (W151/C154G LacY) and pyrene labeled phospholipids (Pyr-PE and Pyr-PG); and (ii) pulling the protein from the supported lipid bilayers where it is embedded by using the tip of the atomic force microscope (AFM). On one hand, fluorescence measurements at different pHs indicate that LacY present selectivity for PE. On the other hand the observations of the reconstituted protein in lipid bilayers by AFM show a preference of LacY for the fluid phase (Lα) rather than for the gel phase (Lβ). To get an estimation of the proportion of each lipid in each phase we have constructed a phase diagram for the system POPE:POPG. The diagram shows that at the temperature of the experiments (24 ºC) there is an almost equimolar proportion of each lipid. The results suggest the existence of a boundary region around LacY formed mainly by POPE laterally segregated from a bulk with a random distribution of POPE and POPG. Force spectroscopy allows to establish the force required and the mechanism to unspecifically unfold the protein.