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Title: | Poly(ADP-ribose) Polymerase 1 (PARP1) restrains MyoD-dependent gene expression during muscle differentiation |
Author: | Matteini, Francesca Andresini, Oriella Petrai, Stefano Battistelli, Cecilia Rossi, Marianna Nicoletta Maione, Rossella |
Keywords: | Múscul estriat Miogènesi Striated muscle Myogenesis |
Issue Date: | 15-Sep-2020 |
Publisher: | Nature Research |
Abstract: | The myogenic factor MyoD regulates skeletal muscle differentiation by interacting with a variety of chromatin-modifying complexes. Although MyoD can induce and maintain chromatin accessibility at its target genes, its binding and trans-activation ability can be limited by some types of not fully characterized epigenetic constraints. In this work we analysed the role of PARP1 in regulating MyoD-dependent gene expression. PARP1 is a chromatin-associated enzyme, playing a well recognized role in DNA repair and that is implicated in transcriptional regulation. PARP1 affects gene expression through multiple mechanisms, often involving the Poly(ADP-ribosyl)ation of chromatin proteins. In line with PARP1 down-regulation during differentiation, we observed that PARP1 depletion boosts the up-regulation of MyoD targets, such as p57, myogenin, Mef2C and p21, while its re-expression reverts this effect. We also found that PARP1 interacts with some MyoD-binding regions and that its presence, independently of the enzymatic activity, interferes with MyoD recruitment and gene induction. We finally suggest a relationship between the binding of PARP1 and the loss of the activating histone modification H3K4me3 at MyoD-binding regions. This work highlights not only a novel player in the epigenetic control of myogenesis, but also a repressive and catalytic-independent mechanisms by which PARP1 regulates transcription. |
Note: | Reproducció del document publicat a: https://doi.org/10.1038/s41598-020-72155-8 |
It is part of: | Scientific Reports, 2020, vol. 10 |
URI: | http://hdl.handle.net/2445/173367 |
Related resource: | https://doi.org/10.1038/s41598-020-72155-8 |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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MatteiniF.pdf | 3.28 MB | Adobe PDF | View/Open |
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