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http://hdl.handle.net/2445/177587
Title: | Regulated secretion is impaired in AtT-20 endocrine cells stably transfected with botulinum neurotoxin type A light chain |
Author: | Aguado Tomàs, Fernando Gombau, Lourdes Majó, Glòria Marsal Tebé, Jordi Blanco Fernández, Jerónimo Blasi Cabús, Joan |
Keywords: | Toxina botulínica Proteïnes de membrana Teixit nerviós Hormones pituïtàries Botulinum toxin Membrane proteins Nerve tissue Pituitary hormones |
Issue Date: | 10-Oct-1997 |
Publisher: | American Society for Biochemistry and Molecular Biology |
Abstract: | Botulinum neurotoxin type A (BoNT/A) inhibits neurotransmitter release by specific cleavage of SNAP-25, a synaptosome-associated protein also expressed in the ACTH secretory cell line AtT-20. Expression of light chain BoNT/A (L-BoNT/A) gene transfected into AtT-20 cells resulted in a cleaved form of SNAP-25 indistinguishable from that generated by bona fide BoNT/A. L-BoNT/A-transfected cells showed no difference in replication rate, viability, or phenotype, compared with control AtT-20 cells. In contrast, L-BoNT/A-transfected cells could not be induced to secrete ACTH upon stimulation by 8-bromo-cAMP or KCl. In addition, alpha-latrotoxin induced ACTH release from control cells, but not from L-BoNT/A-transfected cells. These experiments suggest an important role for SNAP-25 in regulated secretion from AtT-20 cells and underline the usefulness of this cell system as a tool for the study of the molecular mechanism of peptide hormone secretion. |
Note: | Reproducció del document publicat a: https://doi.org/10.1074/jbc.272.41.26005 |
It is part of: | Journal of Biological Chemistry, 1997, vol. 272, num. 41, p. 26005-26008 |
URI: | http://hdl.handle.net/2445/177587 |
Related resource: | https://doi.org/10.1074/jbc.272.41.26005 |
ISSN: | 0021-9258 |
Appears in Collections: | Articles publicats en revistes (Patologia i Terapèutica Experimental) Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia) |
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