Carregant...
Tipus de document
ArticleVersió
Versió publicadaData de publicació
Llicència de publicació
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/184461
PP2A-Cdc55 phosphatase regulates actomyosin ring contraction and septum formation during cytokinesis
Títol de la revista
Director/Tutor
ISSN de la revista
Títol del volum
Resum
Eukaryotic cells divide and separate all their components after chromosome segregation by a process called cytokinesis to complete cell division. Cytokinesis is highly regulated by the recruitment of the components to the division site and through post-translational modifications such as phosphorylations. The budding yeast mitotic kinases Cdc28-Clb2, Cdc5, and Dbf2-Mob1 phosphorylate several cytokinetic proteins contributing to the regulation of cytokinesis. The PP2A-Cdc55 phosphatase regulates mitosis counteracting Cdk1- and Cdc5-dependent phosphorylation. This prompted us to propose that PP2A-Cdc55 could also be counteracting the mitotic kinases during cytokinesis. Here we show that in the absence of Cdc55, AMR contraction and the primary septum formation occur asymmetrically to one side of the bud neck supporting a role for PP2A-Cdc55 in cytokinesis regulation. In addition, by in vivo and in vitro assays, we show that PP2A-Cdc55 dephosphorylates the chitin synthase II (Chs2 in budding yeast) a component of the Ingression Progression Complexes (IPCs) involved in cytokinesis. Interestingly, the non-phosphorylable version of Chs2 rescues the asymmetric AMR contraction and the defective septa formation observed in cdc55 increment mutant cells. Therefore, timely dephosphorylation of the Chs2 by PP2A-Cdc55 is crucial for proper actomyosin ring contraction. These findings reveal a new mechanism of cytokinesis regulation by the PP2A-Cdc55 phosphatase and extend our knowledge of the involvement of multiple phosphatases during cytokinesis.
Descripció
Matèries
Matèries (anglès)
Citació
Citació
MOYANO RODRÍGUEZ, Yolanda, VAQUERO PELAZ, David, VILALTA CASTANY, Odena, FOLTMAN, Magdalena, SÁNCHEZ DÍAZ, Alberto, QUERALT BADIA, Ethel. PP2A-Cdc55 phosphatase regulates actomyosin ring contraction and septum formation during cytokinesis. _Cellular and Molecular Life Sciences_. 2022. Vol. vol 79, núm. num 3. [consulta: 10 de desembre de 2025]. ISSN: 1420-9071. [Disponible a: https://hdl.handle.net/2445/184461]