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Title: In vivo and in vitro characterization of a Plasmodium liver stage-specific promoter
Author: Niz, Mariana De
Helm, Susanne
Horstmann, Sebastian
Annoura, Takeshi
Portillo Obando, Hernando A. del
Khan, Shahid M.
Heussler, Volker T.
Keywords: Plasmodium falciparum
Regulació genètica
Plasmodium falciparum
Genetic regulation
Issue Date: 15-Apr-2015
Publisher: Public Library of Science (PLoS)
Abstract: Little is known about stage-specific gene regulation in Plasmodium parasites, in particular the liver stage of development. We have previously described in the Plasmodium berghei rodent model, a liver stage-specific (lisp2) gene promoter region, in vitro. Using a dual luminescence system, we now confirm the stage specificity of this promoter region also in vivo. Furthermore, by substitution and deletion analyses we have extended our in vitro characterization of important elements within the promoter region. Importantly, the dual luminescence system allows analyzing promoter constructs avoiding mouse-consuming cloning procedures of transgenic parasites. This makes extensive mutation and deletion studies a reasonable approach also in the malaria mouse model. Stage-specific expression constructs and parasite lines are extremely valuable tools for research on Plasmodium liver stage biology. Such reporter lines offer a promising opportunity for assessment of liver stage drugs, characterization of genetically attenuated parasites and liver stage-specific vaccines both in vivo and in vitro, and may be key for the generation of inducible systems.
Note: Reproducció del document publicat a:
It is part of: PLoS One, 2015, vol. 10, num. 4, p. e0123473
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ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (ISGlobal)

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