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2023_AnalChem_Enzymatic_EillsJ.pdf (4.02 MB)

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2023-12-04

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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/209822

Enzymatic Reactions Observed with Zero- and Low-Field Nuclear Magnetic Resonance

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https://doi.org/10.1021/acs.analchem.3c02087

Resum

We demonstrate that enzyme-catalyzed reactions can be observed in zero- and low-field NMR experiments by combining recent advances in parahydrogen-based hyperpolarization methods with state-of-the-art magnetometry. Specifically, we investigated two model biological processes: the conversion of fumarate into malate, which is used in vivo as a marker of cell necrosis, and the conversion of pyruvate into lactate, which is the most widely studied metabolic process in hyperpolarization-enhanced imaging. In addition to this, we constructed a microfluidic zero-field NMR setup to perform experiments on microliter-scale samples of [1-C-13]-fumarate in a lab-on-a-chip device. Zero- to ultralow-field (ZULF) NMR has two key advantages over high-field NMR: the signals can pass through conductive materials (e.g., metals), and line broadening from sample heterogeneity is negligible. To date, the use of ZULF NMR for process monitoring has been limited to studying hydrogenation reactions. In this work, we demonstrate this emerging analytical technique for more general reaction monitoring and compare zero- vs low-field detection.

Matèries

Ressonància magnètica, Hidrogenació

Matèries (anglès)

Magnetic resonance, Hydrogenation

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Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))

Citació

EILLS, James, PICAZO FRUTOS, Román, BONDAR, Oksana, CAVALLARI, Eleonora, CARRERA, Carla, BARKER, Sylwia j, UTZ, Marcel, HERRERO GÓMEZ, Alba, MARCO RIUS, Irene, TAYLER, Michael c. d., AIME, Silvio, REINERI, Francesca, BUDKER, Dmitry, BLANCHARD, John w. Enzymatic Reactions Observed with Zero- and Low-Field Nuclear Magnetic Resonance. _Analytical Chemistry_. 2023. Vol. 95, núm. 49 p. [consulta: 30 de gener de 2026]. ISSN: 0003-2700. [Disponible a: https://hdl.handle.net/2445/209822]

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