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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/209208
Analysis of autophagic vesicles in mitotic cells
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The detection of autophagic vesicles in interphase cells is well characterized with markers such as LC3, SQSTM1 (also known as p62) and LAMP2, which are commonly used in immunofluorescence and biochemistry assays to evaluate the status of autophagy in adherent cells. During mitosis, cells undergo important morphological changes which alter the position of the central plane, therefore the imaging on dividing cells has to be specifically designed. Here, we describe a method to label and image autophagic vesicles in mitotic cells to systematically analyze their number, morphology and distribution.
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ALMACELLAS I CANALS, Eugènia, et al. Analysis of autophagic vesicles in mitotic cells. Autophagy and Cancer. Methods in Molecular Biology. vol 2445. Vol. Norberg, pags. Norberg. ISBN 978-1-0716-2071-7. [consulted: 14 of June of 2026]. Available at: https://hdl.handle.net/2445/209208