Analysis of autophagic vesicles in mitotic cells
| dc.contributor.author | Almacellas i Canals, Eugènia | |
| dc.contributor.author | García Cajide, Marta | |
| dc.contributor.author | Tauler Girona, Albert | |
| dc.contributor.author | Mauvezin, Caroline | |
| dc.date.accessioned | 2024-03-26T17:40:22Z | |
| dc.date.issued | 2022-01-01 | |
| dc.description.abstract | The detection of autophagic vesicles in interphase cells is well characterized with markers such as LC3, SQSTM1 (also known as p62) and LAMP2, which are commonly used in immunofluorescence and biochemistry assays to evaluate the status of autophagy in adherent cells. During mitosis, cells undergo important morphological changes which alter the position of the central plane, therefore the imaging on dividing cells has to be specifically designed. Here, we describe a method to label and image autophagic vesicles in mitotic cells to systematically analyze their number, morphology and distribution. | ca |
| dc.format.extent | 11 p. | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.isbn | 978-1-0716-2071-7 | |
| dc.identifier.uri | https://hdl.handle.net/2445/209208 | |
| dc.language.iso | eng | ca |
| dc.relation.isformatof | Versió postprint del capítol del llibre publicat a: https://doi.org/10.1007/978-1-0716-2071-7_9 | |
| dc.relation.ispartof | Autophagy and Cancer. Methods in Molecular Biology, vol 2445, Norberg, H., Norberg, E. (eds), ISBN: 978-1-0716-2071-7, p. 127-137 | |
| dc.relation.uri | https://doi.org/10.1007/978-1-0716-2071-7_9 | |
| dc.rights | (c) Almacellas i Canals et al., 2022 | |
| dc.rights.accessRights | info:eu-repo/semantics/openAccess | ca |
| dc.source | Llibres / Capítols de llibre (Bioquímica i Biomedicina Molecular) | |
| dc.subject.classification | Lisosomes | |
| dc.subject.classification | Autofàgia | |
| dc.subject.other | Lysosomes | |
| dc.subject.other | Autophagy | |
| dc.title | Analysis of autophagic vesicles in mitotic cells | ca |
| dc.type | info:eu-repo/semantics/bookPart | ca |
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