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Development of DNA Aptamers Against <i>Plasmodium falciparum</i> Blood Stages Using Cell-Systematic Evolution of Ligands by EXponential Enrichment.
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"New biomarkers have to be developed in order to increase the performance of current antigen-based malaria rapid diagnosis. Antibody production often involves the use of laboratory animals and is time-consuming and costly, especially when the target is " - ", whose variable antigen expression
complicates the development of long-lived biomarkers. To
circumvent these obstacles, we have applied the Systematic
Evolution of Ligands by EXponential enrichment method to the
rapid identification of DNA aptamers against " - "-infected red
blood cells (pRBCs). Five 70 b-long ssDNA sequences, and their
shorter forms without the flanking PCR primer-binding regions,
have been identified having a highly specific binding of pRBCs
versus non-infected erythrocytes. Structural analysis revealed
G-enriched sequences compatible with the formation of
G-quadruplexes. The selected aptamers recognized intracellular
epitopes with apparent " - "s in the " - "M range in both fixed
and non-fixed saponin-permeabilized pRBCs, improving
>30-fold the pRBC detection in comparison with aptamers
raised against " - " lactate dehydrogenase, the gold standard
antigen for current malaria diagnostic tests. In thin blood
smears of clinical samples the aptamers reported in this work
specifically bound all " - " stages versus non-infected
erythrocytes, and also detected early and late stages of the
human malaria parasites " - ", " - " and " - . The results are
discussed in the context of their potential application in
future malaria diagnostic devices.
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LANTERO, Elena, BELAVILAS TROVAS, Alexandros, BIOSCA, Arnau, RECOLONS, Paula, MOLES MELER, Ernest, SULLEIRO, Elena, ZARZUELA, Francesc, AVALOS PADILLA, Yunuen, RAMÍREZ, Miriam, FERNÀNDEZ BUSQUETS, Xavier. Development of DNA Aptamers Against <i>Plasmodium falciparum</i> Blood Stages Using Cell-Systematic Evolution of Ligands by EXponential Enrichment.. _Journal of biomedical nanotechnology_. 2020. Vol. 16, núm. 3, pàgs. 315-334. [consulta: 13 de gener de 2026]. ISSN: 1550-7033. [Disponible a: https://hdl.handle.net/2445/185018]