Tipus de document
ArticleVersió
Versió acceptadaData de publicació
Tots els drets reservats
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/185018
Development of DNA Aptamers Against <i>Plasmodium falciparum</i> Blood Stages Using Cell-Systematic Evolution of Ligands by EXponential Enrichment.
Títol de la revista
Director/Tutor
ISSN de la revista
Títol del volum
Recurs relacionat
Resum
"New biomarkers have to be developed in order to increase the performance of current antigen-based malaria rapid diagnosis. Antibody production often involves the use of laboratory animals and is time-consuming and costly, especially when the target is " - ", whose variable antigen expression
complicates the development of long-lived biomarkers. To
circumvent these obstacles, we have applied the Systematic
Evolution of Ligands by EXponential enrichment method to the
rapid identification of DNA aptamers against " - "-infected red
blood cells (pRBCs). Five 70 b-long ssDNA sequences, and their
shorter forms without the flanking PCR primer-binding regions,
have been identified having a highly specific binding of pRBCs
versus non-infected erythrocytes. Structural analysis revealed
G-enriched sequences compatible with the formation of
G-quadruplexes. The selected aptamers recognized intracellular
epitopes with apparent " - "s in the " - "M range in both fixed
and non-fixed saponin-permeabilized pRBCs, improving
>30-fold the pRBC detection in comparison with aptamers
raised against " - " lactate dehydrogenase, the gold standard
antigen for current malaria diagnostic tests. In thin blood
smears of clinical samples the aptamers reported in this work
specifically bound all " - " stages versus non-infected
erythrocytes, and also detected early and late stages of the
human malaria parasites " - ", " - " and " - . The results are
discussed in the context of their potential application in
future malaria diagnostic devices.
Matèries
Matèries (anglès)
Citació
Citació
LANTERO, Elena, et al. Development of DNA Aptamers Against Plasmodium falciparum Blood Stages Using Cell-Systematic Evolution of Ligands by EXponential Enrichment. Journal of biomedical nanotechnology. 2020. Vol. 16, num. 3, pags. 315-334. ISSN 1550-7033. [consulted: 21 of May of 2026]. Available at: https://hdl.handle.net/2445/185018