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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/101682
Peptide aromatic interactions modulated by fluorinated residues: Synthesis, structure and biological activity of Somatostatin analogs containing 3-(3',5'difluorophenyl)-alanine
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Somatostatin is a 14-residue peptide hormone that regulates the endocrine system by binding to five G-protein-coupled receptors (SSTR1-5). We have designed six new Somatostatin analogs with L-3-(3',5'-difluorophenyl)-alanine (Dfp) as a substitute of Phe and studied the effect of an electron-poor arom. ring in the network of arom. interactions present in Somatostatin. Replacement of each of the Phe residues (positions 6, 7 and 11) by Dfp and use of a D-Trp8 yielded peptides whose main conformations could be characterized in aq. soln. by NMR. Receptor binding studies revealed that the analog with Dfp at position 7 displayed a remarkable affinity to SSTR2 and SSTR3. Analogs with Dfp at positions 6 or 11 displayed a π-π interaction with the Phe present at 11 or 6, resp. Interestingly, these analogs, particularly [D-Trp8,L-Dfp11]-SRIF, showed high selectivity towards SSTR2, with a higher value than that of Octreotide and a similar one to that of native Somatostatin.
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MARTÍN-GAGO, Pablo, ROL RÚA, Álvaro, TODOROVSKI, Toni, ARAGÓN ALTARRIBA, Eric, MARTIN-MALPARTIDA, Pau, VERDAGUER I ESPAULELLA, Xavier, VALLÈS MIRET, Mariona, FERNÁNDEZ-CARNEADO, Jimena, PONSATI, Berta, MACÍAS HERNÁNDEZ, María j., RIERA I ESCALÉ, Antoni. Peptide aromatic interactions modulated by fluorinated residues: Synthesis, structure and biological activity of Somatostatin analogs containing 3-(3',5'difluorophenyl)-alanine. _Scientific Reports_. 2016. Vol. 6, núm. 27285. [consulta: 21 de gener de 2026]. ISSN: 2045-2322. [Disponible a: https://hdl.handle.net/2445/101682]