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2019-07-19

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cc-by (c) Marín, Sandra et al., 2019
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/164827

Pancreatic ductal cells may have a negative effect on human islet transplantation. PLoS One. 2019 Jul 19;14(7):e0220064.

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https://doi.org/10.1371/journal.pone.0220064

Resum

Aim: To evaluate the effect of pancreatic ductal cells on experimental human islet transplantation. Materials and methods: Isolated islets were additionally purified by handpicking. Ductal cells were purified by magnetic cell sorting and then clustered into ductal pancreatospheres (DPS). Islets, DPS, and islets + DPS (100 islets + 75 DPS, or 100 islets + 200 DPS) were cultured and glucose-stimulated insulin secretion, β-cell apoptosis, and gene expression was determined. Islets and islets + DPS preparations (800 islets + 600 DPS) were transplanted to streptozotocin-treated immunodeficient mice and glycemia, graft morphometry, and gene expression were determined. Results: Insulin stimulation index was higher in islets than in islets co-cultured with DPS (5.59 ± 0.93 vs 4.02 ± 0.46; p<0.05). IL1B and CXCL11 expression was higher in 100 islets + 200 DPS than in islets (p<0.01), and IL-1β was detected in supernatants collected from DPS and islets + DPS preparations, but not in islets. Hyperglycemia developed in 33% and 67% of mice transplanted with islets or with islets + DPS respectively. β-cell mass was 26% lower in islets + DPS than in islets grafts (p>0.05), and the ratio β-/endocrine non-β-cell mass was lower in islets + DPS grafts (islets: 2.05 ± 0.18, islets + DPS: 1.35 ± 0.15; p<0.01). IL1B and IL1RN expression was significantly higher in islets + DPS grafts. Conclusions: Islet preparations enriched with ductal cells have a lower insulin stimulation index in vitro and achieved a worse metabolic outcome after transplantation. Inflammation may mediate the deleterious effects of ductal cells on islet cells.

Matèries

Illots de Langerhans, Insulina

Matèries (anglès)

Islands of Langerhans, Insulin

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Col·leccions

Articles publicats en revistes (Ciències Clíniques)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

Citació

MARÍN, Sandra, et al. Pancreatic ductal cells may have a negative effect on human islet transplantation. PLoS One. 2019 Jul 19;14(7):e0220064. PLoS One. 2019. Vol. 14, num. 7, pags. e0220064. ISSN 1932-6203. [consulted: 11 of August of 2026]. Available at: https://hdl.handle.net/2445/164827

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