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2023-01-01

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cc-by-nc-nd (c) Elsevier B.V., 2023
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/198462

On the uptake of cationic liposomes by cells: from changes in elasticity to internalization

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https://doi.org/10.1016/j.colsurfb.2022.112968

Abstract

In this study, we assessed the capacity of a previously reported engineered liposomal formulation, which had been tested against model membranes mimicking the lipid composition of the HeLa plasma membrane, to fuse and function as a nanocarrier in cells. We used atomic force microscopy to observe physicochemical changes on the cell surface and confocal microscopy to determine how the liposomes interact with cell membranes and released their load. In addition, we performed viability assays using methotrexate as an active drug to obtain proof of concept of the formulation´s capacity to function as a drug delivery-system. The interaction of engineered liposomes with living cells corroborates the information obtained using model membranes and supports the capacity of the engineered liposomal formulation to serve as a potential nanocarrier.

Subject

Liposomes, Sistemes d'alliberament de medicaments, Microscòpia de força atòmica

Subject (English)

Liposomes, Drug delivery systems, Atomic force microscopy

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Articles publicats en revistes (Farmàcia, Tecnologia Farmacèutica i Fisicoquímica)
Articles publicats en revistes (Bioquímica i Biomedicina Molecular)
Articles publicats en revistes (Bioquímica i Fisiologia)
Articles publicats en revistes (Centres Científics i Tecnològics de la Universitat de Barcelona (CCiTUB))
Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)
Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
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Citation

BOTET CARRERAS, Adrià, et al. On the uptake of cationic liposomes by cells: from changes in elasticity to internalization. Colloids and Surfaces B-Biointerfaces. 2023. Vol. 221, num. 112968. ISSN 0927-7765. [consulted: 11 of August of 2026]. Available at: https://hdl.handle.net/2445/198462

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