Carregant...
Fitxers
Tipus de document
ArticleVersió
Versió publicadaData de publicació
Llicència de publicació
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/219742
Correction to: Molecular basis for bacterial N-glycosylation by a soluble HMW1C-like N-glycosyltransferase (Nature Communications, (2023), 14, 1, (5785), 10.1038/s41467-023-41238-1)
Títol de la revista
Director/Tutor
ISSN de la revista
Títol del volum
Recurs relacionat
Resum
Soluble HMW1C-like N-glycosyltransferases (NGTs) catalyze the glycosylation of Asn residues in proteins, a process fundamental for bacterial autoaggregation, adhesion and pathogenicity. However, our understanding of their molecular mechanisms is hindered by the lack of structures of enzymatic complexes. Here, we report structures of binary and ternary NGT complexes of Aggregatibacter aphrophilus NGT (AaNGT), revealing an essential dyad of basic/acidic residues located in the N-terminal all α-domain (AAD) that intimately recognizes the Thr residue within the conserved motif Asn0-X+1-Ser/Thr+2. Poor substrates and inhibitors such as UDP-galactose and UDP-glucose mimetics adopt non-productive conformations, decreasing or impeding catalysis. QM/MM simulations rationalize these results, showing that AaNGT follows a SN2 reaction mechanism in which the acceptor asparagine uses its imidic form for catalysis and the UDP-glucose phosphate group acts as a general base. These findings provide key insights into the mechanism of NGTs and will facilitate the design of structure-based inhibitors to treat diseases caused by non-typeable H. influenzae or other Gram-negative bacteria.
Matèries
Matèries (anglès)
Citació
Citació
PINIELLO, Beatriz, MACÍAS-LEÓN, Javier, MIYAZAKI, Shun, GARCÍA-GARCÍA, Abel, COMPAÑÓN, Ismael, GHIRARDELLO, Mattia, TALEB, Víctor, VELOZ, Billy, CORZANA, Francisco, MIYAGAWA, Atsushi, ROVIRA I VIRGILI, Carme, HURTADO-GUERRERO, Ramon. Correction to: Molecular basis for bacterial N-glycosylation by a soluble HMW1C-like N-glycosyltransferase (Nature Communications, (2023), 14, 1, (5785), 10.1038/s41467-023-41238-1). _Nature Communications_. 2024. Vol. 15, núm. 1. [consulta: 10 de gener de 2026]. ISSN: 2041-1723. [Disponible a: https://hdl.handle.net/2445/219742]