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cc by (c) Wert Carvajal, Carlos et al., 2021
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/178832

Predicting MHC I restricted T cell epitopes in mice with NAP-CNB, a novel online tool

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Lack of a dedicated integrated pipeline for neoantigen discovery in mice hinders cancer immunotherapy research. Novel sequential approaches through recurrent neural networks can improve the accuracy of T-cell epitope binding affinity predictions in mice, and a simplified variant selection process can reduce operational requirements. We have developed a web server tool (NAP-CNB) for a full and automatic pipeline based on recurrent neural networks, to predict putative neoantigens from tumoral RNA sequencing reads. The developed software can estimate H-2 peptide ligands, with an AUC comparable or superior to state-of-the-art methods, directly from tumor samples. As a proof-of-concept, we used the B16 melanoma model to test the system's predictive capabilities, and we report its putative neoantigens. NAP-CNB web server is freely available at http://biocomp.cnb.csic.es/NeoantigensApp/ with scripts and datasets accessible through the download section.

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WERT CARVAJAL, Carlos, et al. Predicting MHC I restricted T cell epitopes in mice with NAP-CNB, a novel online tool. Scientific Reports. 2021. Vol. 11, num. 10780. [consulted: 17 of August of 2026]. Available at: https://hdl.handle.net/2445/178832

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