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Isoprenoid biosynthesis in Escherichia coli via the methylerythritol phosphate pathway: enzymatic conversion of methylerythritol cyclodiphosphate into a phosphorylated derivative of (E)-2-methylbut-2-ene-1,4-diol

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dc.contributor.authorSeemann, Myriam
dc.contributor.authorCampos Martínez, Narciso
dc.contributor.authorRodríguez Concepción, Manuel
dc.contributor.authorIbañez, Esther
dc.contributor.authorDuvold, Tore
dc.contributor.authorTritsch, Denis
dc.contributor.authorBoronat i Margosa, Albert
dc.contributor.authorRohmer, Michel
dc.date.accessioned2026-01-22T07:55:40Z
dc.date.available2026-01-22T07:55:40Z
dc.date.issued2002-02-18
dc.date.updated2026-01-22T07:55:40Z
dc.description.abstractA crude cell-free system from an Escherichia coli strain overexpressing the cluster containing the three genes yfgA, yfgB, and gcpE converted 2-C-methyl-d-erythritol 2,4-cyclodiphosphate (1) into a phosphorylated derivative of (E)-2-methylbut-2-ene-1,4-diol (6), which most probably represents a novel intermediate in the methylerythritol phosphate pathway for isoprenoid biosynthesis. The free diol 6 was accumulated by phosphatase treatment of the crude enzyme preparation and was identified by comparison with a synthetic reference.
dc.format.extent3 p.
dc.format.mimetypeapplication/pdf
dc.identifier.idgrec511478
dc.identifier.issn0040-4039
dc.identifier.urihttps://hdl.handle.net/2445/225926
dc.language.isoeng
dc.publisherElsevier Ltd.
dc.relation.isformatofVersió postprint del document publicat a: https://doi.org/10.1016/S0040-4039(02)00003-5
dc.relation.ispartofTetrahedron Letters, 2002, vol. 43, num.8, p. 1413-1415
dc.relation.urihttps://doi.org/10.1016/S0040-4039(02)00003-5
dc.rights(c) Elsevier Ltd., 2002
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.sourceArticles publicats en revistes (Bioquímica i Biomedicina Molecular)
dc.subject.classificationEscheríchia coli
dc.subject.classificationEnzims microbians
dc.subject.otherEscherichia coli
dc.subject.otherMicrobial enzymes
dc.titleIsoprenoid biosynthesis in Escherichia coli via the methylerythritol phosphate pathway: enzymatic conversion of methylerythritol cyclodiphosphate into a phosphorylated derivative of (E)-2-methylbut-2-ene-1,4-diol
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/acceptedVersion

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