Amb motiu del tancament d'estiu, la validació de documents es reprendrà a partir del 28 d'agost de 2026. Disculpeu les molèsties.
Con motivo del cierre de verano, la validación de documentos se reanudará a partir del 28 de agosto de 2026. Disculpad las molestias
Due to the summer closure, document validation will resume starting August 28, 2026. We apologize for any inconvenience.

Files

2023_Nan_Asuper_AlbertazziL.pdf (1.69 MB)

Document type

Article

Version

Published version

Publication date

2023-08-14

Publication license

cc by-nc (c) Andrian, Teodora et al., 2023
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/202063

A super-resolution and transmission electron microscopy correlative approach to study intracellular trafficking of nanoparticles

Journal Title

Authors

Director/Tutor

Journal ISSN

Volume Title

Related resource

https://doi.org/10.1039/d3nr02838k

Abstract

Nanoparticles (NPs) are used to encapsulate therapeutic cargos and deliver them specifically to the target site. The intracellular trafficking of NPs dictates the NP-cargo distribution within different cellular compartments, and thus governs their efficacy and safety. Knowledge in this field is crucial to understand their biological fate and improve their rational design. However, there is a lack of methods that allow precise localization and quantification of individual NPs within distinct cellular compartments simultaneously. Here, we address this issue by proposing a correlative light and electron microscopy (CLEM) method combining direct stochastic optical reconstruction microscopy (dSTORM) and transmission electron microscopy (TEM). We aim at combining the advantages of both techniques to precisely address NP localization in the context of the cell ultrastructure. Individual fluorescently-labelled poly(lactide-co-glycolide)-poly(ethylene glycol) (PLGA-PEG) NPs were directly visualized by dSTORM and assigned to cellular compartments by TEM. We first tracked NPs along the endo-lysosomal pathway at different time points, then demonstrated the effect of chloroquine on their intracellular distribution (i.e. endosomal escape). The proposed protocol can be applied to fluorescently labelled NPs and/or cargo, including those not detectable by TEM alone. Our studies are of great relevance to obtain important information on NP trafficking, and crucial for the design of more complex nanomaterials aimed at cytoplasmic/nucleic drug delivery.

Subject

Nanopartícules, Transport biològic, Microscòpia electrònica de transmissió

Subject (English)

Nanoparticles, Biological transport, Transmission electron microscopy

Citation

Andrian T;Muela Y;Delgado L;Albertazzi L;Pujals S. A super-resolution and transmission electron microscopy correlative approach to study intracellular trafficking of nanoparticles. Nanoscale, 2023, 15, 35, 14615-14627

Collections

Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))

Citation

ANDRIAN, Teodora, et al. A super-resolution and transmission electron microscopy correlative approach to study intracellular trafficking of nanoparticles. Nanoscale. 2023. Vol. 15, num. 35, pags. 14615-14627. ISSN 2040-3372. [consulted: 15 of August of 2026]. Available at: https://hdl.handle.net/2445/202063

Export metadata

JSON - METS

Share record