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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/155979
Differences in peripheral and tissue immune cell populations following haematopoietic stem cell transplantation in Crohn's disease patients
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Background and aims: recent studies have shown the efficacy of autologous haematopoietic stem cell transplantation [HSCT] in severely refractory Crohn's disease [CD] patients. HSCT is thought to eliminate auto-reactive cells; however, no specific studies of immune reconstitution in CD patients are available. Methods: we followed a group of CD patients [n = 18] receiving autologous HSCT, with 50% of them achieving endoscopic drug-free remission. To elucidate the mechanisms driving efficacy, we monitored changes after HSCT in blood and intestine immune-cell composition. CD patients [n = 22] receiving anti-tumour necrosis factor [TNF]-α were included for comparison. Results: severe immune ablation followed by HSCT induced dramatic changes in both peripheral blood T and B cells in all patients regardless of the efficacy of the treatment. Endoscopic remission at week 52 following HSCT was associated with significant intestinal transcriptional changes. A comparison of the remission signature with that of anti-TNFα identified both common and unique genes in the HSCT-induced response. Based on deconvolution analysis of intestinal biopsy transcriptome data, we show that response to HSCT, but not to anti-TNFα, is associated with an expansion of naïve B-cells, as seen in blood, and a decrease in the memory resting T-cell content. As expected, endoscopic remission, in response to both HSCT and anti-TNFα, led to a significant reduction in intestinal neutrophil and M1 macrophage content. Conclusions: peripheral blood immune remodelling after HSCT does not predict efficacy. In contrast, a profound intestinal T-cell depletion that is maintained long after transplant is associated with mucosal healing following HSCT, but not anti-TNFα.
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CORRALIZA MÁRQUEZ, Ana maria, RICART, Elena, LÓPEZ-GARCÍA, Alicia, MASAMUNT, Maria carme, VENY ALVAREZ-OSSORIO, Marisol, ESTELLER, Manel, MAYORGAS, Aida, LE BOURHIS, Lionel, ALLEZ, Matthieu, PLANELL PICOLA, Núria, VISVANATHAN, Sudha, BAUM, Patrick, ESPAÑA, Carolina, CABEZÓN CABELLO, Raquel, BENÍTEZ-RIBAS, Daniel, ROVIRA TARRATS, Montserrat, PANÉS DÍAZ, Julià, SALAS MARTÍNEZ, Azucena. Differences in peripheral and tissue immune cell populations following haematopoietic stem cell transplantation in Crohn's disease patients. _Journal of Crohn's and Colitis_. 2019. Vol. 13, núm. 5, pàgs. 634-647. [consulta: 21 de gener de 2026]. ISSN: 1873-9946. [Disponible a: https://hdl.handle.net/2445/155979]