Document type
ArticleVersion
Published versionPublication date
Publication license
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/182197
Disposable Polymeric Nanostructured Plasmonic Biosensors for Cell Culture Adhesion Monitoring
Journal Title
Director/Tutor
Journal ISSN
Volume Title
Related resource
Abstract
Over the last years, optical biosensors based on plasmonic nanomaterials have gained great scientific interest due to their unquestionable advantages compared to other biosensing technologies. They can achieve sensitive, direct, and label-free analysis with exceptional potential for multiplexing and miniaturization. Recently, it has been demonstrated the potential of using optical discs as high throughput nanotemplates for the development of plasmonic biosensors in a cost-effective way. This work is a pilot study focused on the development of an integrated plasmonic biosensor for the monitoring of cell adhesion and growth of human retinal pigmented cell line (ARPE-19) under different media conditions (0 and 2% of FBS). We observed an increase of the plasmonic band displacement under 2% FBS compared to 0% conditions over time (1, 3, and 5 h). These preliminary results show that the proposed plasmonic biosensing approach is a direct, non-destructive, and real-time tool that could be employed in the study of living cells behavior and culture conditions. Furthermore, this setup could assess the viability of the cells and their growth over time with low variability between the technical replicates improving the experimental replicability.
Subject
Subject (English)
Citation
Citation
CAMALÓ VILA, Judith, et al. Disposable Polymeric Nanostructured Plasmonic Biosensors for Cell Culture Adhesion Monitoring. Frontiers In Bioengineering And Biotechnology. 2021. Vol. 9. ISSN 2296-4185. [consulted: 7 of August of 2026]. Available at: https://hdl.handle.net/2445/182197