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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/109007
Sugar nucleotide quantification by liquid chromatography tandem mass spectrometry reveals a distinct profile in Plasmodium falciparum sexual stage parasites
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Abstract
The obligate intracellular lifestyle of Plasmodium falciparum
and the difficulties to obtain sufficient amounts of biological
material has hampered the study of specific metabolic pathways
in the malaria parasite. Thus, for example, the pools of sugar
nucleotides required to fuel glycosylation reactions have never
been studied in-depth in well-synchronized asexual parasites or
in other stages of its life cycle. These metabolites are of
critical importance, especially considering the renewed interest
in the presence of N -, O - and other glycans in key parasite
proteins. In this work, we adapted a liquid chromatography
tandem mass spectrometry method based on the use of porous
graphitic carbon (PGC) columns and MS-friendly solvents to
quantify sugar nucleotides in the malaria parasite. We report on
the thorough quantification of the pools of these metabolites
throughout the intraerythrocytic cycle of P. falciparum The
sensitivity of the method enabled for the first time the
targeted analysis of these glycosylation precursors in
gametocytes, the parasite sexual stages that are transmissible
to the mosquito vector.
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LÓPEZ GUTIÉRREZ, Borja, DINGLASAN, Rhoel R. and IZQUIERDO LÁZARO, Luis. Sugar nucleotide quantification by liquid chromatography tandem
mass spectrometry reveals a distinct profile in Plasmodium
falciparum sexual stage parasites. The Biochemical Journal. 2017. Vol. 6, num. 474, pags. 897-905. ISSN 0264-6021. [consulted: 16 of May of 2026]. Available at: https://hdl.handle.net/2445/109007