Carregant...
Tipus de document
ArticleVersió
Versió acceptadaData de publicació
Tots els drets reservats
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/197645
The Relation Between Protein Adsorption and Hemocompatibility of Antifouling Polymer Brushes
Títol de la revista
Director/Tutor
ISSN de la revista
Títol del volum
Recurs relacionat
Resum
Whenever an artificial surface comes into contact with blood, proteins are rapidly adsorbed onto its surface. This phenomenon, termed fouling, is then followed by a series of undesired reactions involving activation of complement or the coagulation cascade and adhesion of leukocytes and platelets leading to thrombus formation. Thus, considerable efforts are directed towards the preparation of fouling-resistant surfaces with the best possible hemocompatibility. Herein, a comprehensive hemocompatibility study after heparinized blood contact with seven polymer brushes prepared by surface-initiated atom transfer radical polymerization is reported. The resistance to fouling is quantified and thrombus formation and deposition of blood cellular components on the coatings are analyzed. Moreover, identification of the remaining adsorbed proteins is performed via mass spectroscopy to elucidate their influence on the surface hemocompatibility. Compared with an unmodified glass surface, the grafting of polymer brushes minimizes the adhesion of platelets and leukocytes and prevents the thrombus formation. The fouling from undiluted blood plasma is reduced by up to 99%. Most of the identified proteins are connected with the initial events of foreign body reaction towards biomaterial (coagulation cascade proteins, complement component, and inflammatory proteins). In addition, several proteins that are not previously linked with blood-biomaterial interaction are presented and discussed.
Matèries
Matèries (anglès)
Citació
Citació
RIEDELOVÁ, Zuzana, SANTOS PEREIRA, Andres de los, SVOBODA, Jan, POP GEORGIEVSKI, Ognen, MÁJEK, Pavel, PEČÁNKOVÁ, Klára, DYČKA, Filip, RODRIGUEZ EMMENEGGER, César, RIEDEL, Tomáš. The Relation Between Protein Adsorption and Hemocompatibility of Antifouling Polymer Brushes. _Macromolecular Bioscience_. 2022. Vol. 22, núm. 11, pàgs. 2200247. [consulta: 27 de febrer de 2026]. ISSN: 1616-5195. [Disponible a: https://hdl.handle.net/2445/197645]