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2018-05-27

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cc-by (c) Álvarez, Claudio Andrés et al., 2018
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/156081

Identification of Antimicrobial Peptides in Flowers of Sambucus Nigra

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https://doi.org/10.3390/molecules23051033

Abstract

The elder (Sambucus spp.) tree has a number of uses in traditional medicine. Previous studies have demonstrated the antimicrobial properties of elderberry liquid extract against human pathogenic bacteria and also influenza viruses. These properties have been mainly attributed to phenolic compounds. However, other plant defense molecules, such as antimicrobial peptides (AMPs), may be present. Here, we studied peptide extracts from flowers of Sambucus nigra L. The mass spectrometry analyses determined peptides of 3 to 3.6 kDa, among them, cysteine-rich peptides were identified with antimicrobial activity against various Gram-negative bacteria, including recurrent pathogens of Chilean aquaculture. In addition, membrane blebbing on the bacterial surface after exposure to the cyclotide was visualized by SEM microscopy and SYTOX Green permeabilization assay showed the ability to disrupt the bacterial membrane. We postulate that these peptides exert their action by destroying the bacterial membrane.

Subject

Medicina tradicional, Agents antiinfecciosos, Influenzavirus, Medicaments peptídics, Microorganismes patògens

Subject (English)

Traditional medicine, Anti-infective agents, Influenza viruses, Peptide drugs, Pathogenic microorganisms

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Articles publicats en revistes (Química Inorgànica i Orgànica)

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ÁLVAREZ, Claudio Andrés, et al. Identification of Antimicrobial Peptides in Flowers of Sambucus Nigra. Molecules. 2018. Vol. 23, num. 5, pags. 1033. ISSN 1420-3049. [consulted: 11 of June of 2026]. Available at: https://hdl.handle.net/2445/156081

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