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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/110939
2-picolylamine derivatization for high sensitivity detection of abscisic acid in apicomplexan blood-infecting parasites
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We have developed a new liquid chromatography-electrospray
ionization tandem mass spectrometry methodology based on
2-picolylamine derivatization and positive ion mode detection
for abscisic acid (ABA) identification. The selected reaction
leads to the formation of an amide derivative which contains a
highly active pyridyl group. The enhanced ionization allows for
a 700-fold increase over commonly monitored unmodified ABA,
which in turn leads to excellent limits of detection and
quantification values of 0.03 and 0.15ngmL-1, respectively. This
method has been validated in the highly complex matrix of a red
blood cell extract. In spite of the high sensitivity achieved,
ABA could not be detected in Plasmodium falciparum-infected red
blood cells, suggesting that, if present, it will be found
either in ultratrace amounts or as brief bursts at defined time
points within the intraerythrocytic cycle and/or in the form of
a biosynthetic analogue.
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MOLES MELER, Ernest, MARCOS, Josep, IMPERIAL RÓDENAS, Santiago, POZO, Oscar j., FERNÀNDEZ BUSQUETS, Xavier. 2-picolylamine derivatization for high sensitivity detection of
abscisic acid in apicomplexan blood-infecting parasites. _Talanta_. 2017. Vol. 168, núm. 130-135. [consulta: 31 de gener de 2026]. ISSN: 0039-9140. [Disponible a: https://hdl.handle.net/2445/110939]