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2020-09-29

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cc-by (c) Cuatrecasas Freixas, Miriam et al., 2020
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/173916

Complete Loss of EPCAM Immunoexpression Identifies EPCAM Deletion Carriers in MSH2-Negative Colorectal Neoplasia

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https://doi.org/10.3390/cancers12102803

Resum

The use of epithelial cell adhesion molecule (EPCAM) immunohistochemistry (IHC) is not included in the colorectal cancer (CRC) screening algorithm to detect Lynch syndrome (LS) patients. The aim of the present study was to demonstrate that EPCAM IHC is a useful tool to guide the LS germ-line analysis when a loss of MSH2 expression was present. We retrospectively studied MSH2 and EPCAM IHC in a large series of 190 lesions composed of malignant neoplasms (102), precursor lesions of gastrointestinal (71) and extra-gastrointestinal origin (9), and benign neoplasms (8) from different organs of 71 patients suspicious of being LS due to MSH2 alterations. LS was confirmed in 68 patients, 53 with MSH2 mutations and 15 with EPCAM 3'-end deletions. Tissue microarrays were constructed with human normal tissues and their malignant counterparts to assist in the evaluation of EPCAM staining. Among 154 MSH2-negative lesions, 17 were EPCAM-negative, including 10 CRC and 7 colorectal polyps, and 5 of them showed only isolated negative glands. All lesions showing a lack of EPCAM expression belonged to patients with EPCAM 3'-end deletions. EPCAM IHC is a useful screening tool, with 100% specificity to identify LS patients due to EPCAM 3'-end deletions in MSH2-negative CRC and MSH2-negative colorectal polyps.

Matèries

Càncer colorectal, Malalties del còlon, Immunohistoquímica, Pòlips (Patologia)

Matèries (anglès)

Colorectal cancer, Colonic diseases, Immunohistochemistry, Polyps (Pathology)

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Articles publicats en revistes (Ciències Fisiològiques)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

Citació

CUATRECASAS FREIXAS, Miriam, GOROSTIAGA, Iñigo, RIERA, Cristina, SAPERAS, Esteban, LLORT, Gemma, COSTA, Irmgard, MATIAS-GUIU, Xavier, CARRATO, Cristina, NAVARRO, Matilde, PINEDA RIU, Marta, DUEÑAS, Nuria, BRUNET, Joan, MARCO, Vicente, TRIAS, Isabel, BUSTEROS, José ignacio, MATEU, Gemma, BALAGUER PRUNÉS, Francesc, FERNÁNDEZ FIGUERAS, María teresa, ESTELLER, Manel, MUSULÉN, Eva. Complete Loss of EPCAM Immunoexpression Identifies EPCAM Deletion Carriers in MSH2-Negative Colorectal Neoplasia. _Cancers_. 2020. Vol. 12(10), núm. 2803. [consulta: 25 de gener de 2026]. ISSN: 2072-6694. [Disponible a: https://hdl.handle.net/2445/173916]

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