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Published version

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2024-05

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cc by-nc-nd (c) Martínez San Segundo, P. et al., 2024
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/214841

Protocol to correlate electron microscopy with electrophysiology in single-cell autaptic microcultures

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https://doi.org/10.1016/j.xpro.2024.103003

Abstract

Single-cell microcultures (SCMs) form a monosynaptic circuit that allows stimulation and recording of postsynaptic responses using a single electrode. Here, we present a protocol to establish autaptic cultures from rat superior cervical ganglion neurons. We describe the steps for preparing SCMs, recording synaptic currents, and identifying and processing the recorded neurons for electron microscopy. We then detail procedures for visualizing synapses. This protocol is illustrated by correlating evoked and spontaneous neurotransmitter release with the ultrastructural features of synapses recorded. For complete details on the use and execution of this protocol, please refer to Velasco et al.

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Electrofisiologia, Cèl·lules, Neurones, Rates (Animals de laboratori)

Subject (English)

Electrophysiology, Cells, Neurons, Rats as laboratory animals

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Articles publicats en revistes (Patologia i Terapèutica Experimental)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

Citation

MARTÍNEZ SAN SEGUNDO, Pablo, et al. Protocol to correlate electron microscopy with electrophysiology in single-cell autaptic microcultures. STAR protocols. 2024. Vol. 5, num. 2. [consulted: 16 of August of 2026]. Available at: https://hdl.handle.net/2445/214841

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