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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/199885
Molecular characterization of hepatocellular carcinoma in patients with nonalcoholic steatohepatitis
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Background and aims: Non-alcoholic steatohepatitis (NASH)-related hepatocellular carcinoma (HCC) is increasing globally, but its molecular features are not well defined. We aimed to identify unique molecular traits characterising NASH-HCC compared to other HCC aetiologies. Methods: We collected 80 NASH-HCC and 125 NASH samples from 5 institutions. Expression array (n = 53 NASH-HCC; n = 74 NASH) and whole exome sequencing (n = 52 NASH-HCC) data were compared to HCCs of other aetiologies (n = 184). Three NASH-HCC mouse models were analysed by RNA-seq/expression-array (n = 20). Activin A receptor type 2A (ACVR2A) was silenced in HCC cells and proliferation assessed by colorimetric and colony formation assays. Results: Mutational profiling of NASH-HCC tumours revealed TERT promoter (56%), CTNNB1 (28%), TP53 (18%) and ACVR2A (10%) as the most frequently mutated genes. ACVR2A mutation rates were higher in NASH-HCC than in other HCC aetiologies (10% vs. 3%, p <0.05). In vitro, ACVR2A silencing prompted a significant increase in cell proliferation in HCC cells. We identified a novel mutational signature (MutSig-NASH-HCC) significantly associated with NASH-HCC (16% vs. 2% in viral/alcohol-HCC, p = 0.03). Tumour mutational burden was higher in non-cirrhotic than in cirrhotic NASH-HCCs (1.45 vs. 0.94 mutations/megabase; p <0.0017). Compared to other aetiologies of HCC, NASH-HCCs were enriched in bile and fatty acid signalling, oxidative stress and inflammation, and presented a higher fraction of Wnt/TGF-β proliferation subclass tumours (42% vs. 26%, p = 0.01) and a lower prevalence of the CTNNB1 subclass. Compared to other aetiologies, NASH-HCC showed a significantly higher prevalence of an immunosuppressive cancer field. In 3 murine models of NASH-HCC, key features of human NASH-HCC were preserved. Conclusions: NASH-HCCs display unique molecular features including higher rates of ACVR2A mutations and the presence of a newly identified mutational signature. Lay summary: The prevalence of hepatocellular carcinoma (HCC) associated with non-alcoholic steatohepatitis (NASH) is increasing globally, but its molecular traits are not well characterised. In this study, we uncovered higher rates of ACVR2A mutations (10%) - a potential tumour suppressor - and the presence of a novel mutational signature that characterises NASH-related HCC.
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PINYOL, Roser, TORRECILLA, Sara, WANG, Huan, MONTIRONI, Carla, PIQUÉ GILI, Marta, TORRES MARTÍN, Miguel, WEI-QIANG, Leow, WILLOUGHBY, Catherine e., RAMADORI, Pierluigi, ANDREU OLLER, Carmen, TAIK, Patricia, LEE, Youngmin a., MOEINI, Agrin, PEIX, Judit, FAURE-DUPUY, Suzanne, RIEDL, Tobias, SCHUEHLE, Svenja, OLIVEIRA, Claudia p., ALVES, Venancio a., BOFFETTA, Paolo, LACHENMAYER, Anja, ROESSLER, Sthephanie, MINGUEZ, Beatriz, SCHIRMACHER, Peter, DUFOUR, Jean-françois, THUNG, Swan n., REEVES, Helen l., CARRILHO, Flair j., CHANG, Charissa, UZILOV, Andrew v., HEIKENWÄLDER, Mathias, SANYAL, Arun, FRIEDMAN, Scott l., SIA, Daniela, LLOVET I BAYER, Josep maria. Molecular characterization of hepatocellular carcinoma in patients with nonalcoholic steatohepatitis. _Journal of Hepatology_. 2021. Vol. 75, núm. 4, pàgs. 865-878. [consulta: 23 de gener de 2026]. ISSN: 0168-8278. [Disponible a: https://hdl.handle.net/2445/199885]