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2016-10-05

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cc-by (c) Scheler, Ulschan et al., 2016
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/104313

Elucidation of the biosynthesis of carnosic acid and its reconstitution in yeast

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https://doi.org/10.1038/ncomms12942

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Rosemary extracts containing the phenolic diterpenes carnosic acid and its derivative carnosol are approved food additives used in an increasingly wide range of products to enhance shelf-life, thanks to their high anti-oxidant activity. We describe here the elucidation of the complete biosynthetic pathway of carnosic acid and its reconstitution in yeast cells. Cytochrome P450 oxygenases (CYP76AH22-24) from Rosmarinus officinalis and Salvia fruticosa already characterized as ferruginol synthases are also able to produce 11-hydroxyferruginol. Modelling-based mutagenesis of three amino acids in the related ferruginol synthase (CYP76AH1) from S. miltiorrhiza is sufficient to convert it to a 11-hydroxyferruginol synthase (HFS). The three sequential C20 oxidations for the conversion of 11-hydroxyferruginol to carnosic acid are catalysed by the related CYP76AK6-8. The availability of the genes for the biosynthesis of carnosic acid opens opportunities for the metabolic engineering of phenolic diterpenes, a class of compounds with potent anti-oxidant, anti-inflammatory and anti-tumour activities.

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Additius alimentaris, Llevats (Botànica), Biosíntesi

Matèries (anglès)

Food additives, Yeast fungi, Biosynthesis

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Articles publicats en revistes (Bioquímica i Fisiologia)
<b>Publicacions de projectes de recerca finançats per la UE</b>

Citació

SCHELER, Ulschan, BRANDT, Wolfgang, PORZEL, Andrea, ROTHE, Kathleen, MANZANO ALÍAS, David, BOZIC, Dragana, PAPAEFTHIMIOU, Dimitra, BALCKE, Gerd ulrich, HENNING, Anja, LOHSE, Swanhild, MARILLONNET, Sylvestre, KANELLIS, Angelos k., FERRER I PRATS, Albert, TISSIER, Alain. Elucidation of the biosynthesis of carnosic acid and its reconstitution in yeast. _Nature Communications_. 2016. Vol. 7, núm. 1-11. [consulta: 29 de gener de 2026]. ISSN: 2041-1723. [Disponible a: https://hdl.handle.net/2445/104313]

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