Elucidation of the biosynthesis of carnosic acid and its reconstitution in yeast

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dc.contributor.authorScheler, Ulschan
dc.contributor.authorBrandt, Wolfgang
dc.contributor.authorPorzel, Andrea
dc.contributor.authorRothe, Kathleen
dc.contributor.authorManzano Alías, David
dc.contributor.authorBozic, Dragana
dc.contributor.authorPapaefthimiou, Dimitra
dc.contributor.authorBalcke, Gerd Ulrich
dc.contributor.authorHenning, Anja
dc.contributor.authorLohse, Swanhild
dc.contributor.authorMarillonnet, Sylvestre
dc.contributor.authorKanellis, Angelos K.
dc.contributor.authorFerrer i Prats, Albert
dc.contributor.authorTissier, Alain
dc.date.accessioned2016-11-30T17:35:42Z
dc.date.available2016-11-30T17:35:42Z
dc.date.issued2016-10-05
dc.date.updated2016-11-30T17:35:47Z
dc.description.abstractRosemary extracts containing the phenolic diterpenes carnosic acid and its derivative carnosol are approved food additives used in an increasingly wide range of products to enhance shelf-life, thanks to their high anti-oxidant activity. We describe here the elucidation of the complete biosynthetic pathway of carnosic acid and its reconstitution in yeast cells. Cytochrome P450 oxygenases (CYP76AH22-24) from Rosmarinus officinalis and Salvia fruticosa already characterized as ferruginol synthases are also able to produce 11-hydroxyferruginol. Modelling-based mutagenesis of three amino acids in the related ferruginol synthase (CYP76AH1) from S. miltiorrhiza is sufficient to convert it to a 11-hydroxyferruginol synthase (HFS). The three sequential C20 oxidations for the conversion of 11-hydroxyferruginol to carnosic acid are catalysed by the related CYP76AK6-8. The availability of the genes for the biosynthesis of carnosic acid opens opportunities for the metabolic engineering of phenolic diterpenes, a class of compounds with potent anti-oxidant, anti-inflammatory and anti-tumour activities.
dc.format.extent11 p.
dc.format.mimetypeapplication/pdf
dc.identifier.idgrec664512
dc.identifier.issn2041-1723
dc.identifier.pmid27703160
dc.identifier.urihttps://hdl.handle.net/2445/104313
dc.language.isoeng
dc.publisherNature Publishing Group
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.1038/ncomms12942
dc.relation.ispartofNature Communications, 2016, vol. 7, p. 1-11
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/227448/EU//TERPMED
dc.relation.urihttps://doi.org/10.1038/ncomms12942
dc.rightscc-by (c) Scheler, Ulschan et al., 2016
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es
dc.sourceArticles publicats en revistes (Bioquímica i Fisiologia)
dc.subject.classificationAdditius alimentaris
dc.subject.classificationLlevats (Botànica)
dc.subject.classificationBiosíntesi
dc.subject.otherFood additives
dc.subject.otherYeast fungi
dc.subject.otherBiosynthesis
dc.titleElucidation of the biosynthesis of carnosic acid and its reconstitution in yeast
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion

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