Fitxers
Tipus de document
ArticleVersió
Versió publicadaData de publicació
Llicència de publicació
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/126087
Optical modulation of neurotransmission using calcium photocurrents through the ion channel LiGluR
Títol de la revista
Director/Tutor
ISSN de la revista
Títol del volum
Recurs relacionat
Resum
A wide range of light-activated molecules (photoswitches and phototriggers) have been used to the study of computational properties of an isolated neuron by acting pre and postsynaptically. However, new tools are being pursued to elicit a presynaptic calcium influx that triggers the release of neurotransmitters, most of them based in calcium-permeable Channelrhodopsin-2 mutants. Here we describe a method to control exocytosis of synaptic vesicles through the use of a light-gated glutamate receptor (LiGluR), which has recently been demonstrated that supports secretion by means of calcium influx in chromaffin cells. Expression of LiGluR in hippocampal neurons enables reversible control of neurotransmission with light, and allows modulating the firing rate of the postsynaptic neuron with the wavelength of illumination. This method may be useful for the determination of the complex transfer function of individual synapses.
Matèries
Matèries (anglès)
Citació
Citació
IZQUIERDO SERRA, Mercè, et al. Optical modulation of neurotransmission using calcium photocurrents through the ion channel LiGluR. Frontiers In Molecular Neuroscience. 2013. Vol. 6, núm. 3. ISSN 1662-5099. [consulta: 8 de maig de 2026]. Disponible a: https://hdl.handle.net/2445/126087