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Homogeneous Crystal Nucleation Near a Metastable Fluid-Fluid Phase Transition

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Several scenarios exist for the protein crystallization and aggregation in solutions near a metastable fluid-fluid phase separation below the solubility line. Based on computations, it was proposed that the fluid-fluid critical point enhances the crystallization rate by many orders of magnitude, while, based on experiments, it was proposed that the fluid-fluid spinodal controls the crystallization rate. Using molecular dynamic simulations for an isotropic model with sticky interaction, we show that neither of these scenarios adequately describes the crystallization mechanism near a metastable fluid-fluid phase separation. We find that the emergence of the high-density fluid inside the spinodal drastically enhances the crystal nucleation in the subcritical region following Ostwald's rule of stages.

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XU, Limei, BULDYREV, V. s. (vladimir sergeevich), STANLEY, H. eugene (harry eugene), FRANZESE, Giancarlo. Homogeneous Crystal Nucleation Near a Metastable Fluid-Fluid Phase Transition. _Physical Review Letters_. 2012. Vol. 109, núm. 9, pàgs. 095702. [consulta: 20 de gener de 2026]. ISSN: 0031-9007. [Disponible a: https://hdl.handle.net/2445/133725]

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