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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/225916
Ion mobility–mass spectrometry of palytoxin-like compounds produced by Ostreopsis cf. ovata
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Palytoxin (PLTX) and its analogues from Ostreopsis cf. ovata are significant health concerns. They show potent vasoconstrictive properties, often causing seafood poisoning. PLTX analogues have chiral centers, resulting in many isomers, making their separation by liquid chromatography and identification/characterization by mass spectrometry challenging. This study explores for the first time the ion mobility spectrometry (IMS) behavior of these compounds to address these analytical challenges. Drift tube ion mobility spectrometry (DTIMS) and traveling wave ion mobility spectrometry (TWIMS) were used and compared. Additionally, trapped ion mobility spectrometry (TIMS) and molecular dynamics simulation were employed to explain unexpected results. TIMS provided higher resolution, distinguishing isomeric ions generated in the electrospray source by losing water molecules from different toxin sites. Computational studies offered theoretical insights into the ion mobility of triply charged calcium and sodium adduct ions, suggesting a folded conformation. DTCCSN2 (collisional cross section using DTIMS and nitrogen as buffer gas) values were obtained for PLTX (standard), ovatoxin-a, and ovatoxin-b from microalgae samples in Sant Andreu de Llavaneres (Barcelona, Spain). These values were comparable (ΔCCSs < 2%) to those measured with TWIMS calibrated using PLTX (standard). The study provides 102 CCS values from DTIMS and TWIMS data for adducts and fragment ions of PLTX analogues, which can be used as reference values in databases for toxin screening in complex samples.
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MEDINA PÉREZ, Noemí-inmaculada, PERALTA MORENO, María nuria, RUBIO MARTÍNEZ, Jaime, BECHTELLA, Leïla, POLEWSKI, Lukasz, SZEKERES, Gergo peter, BERDALET, Elisa, MOYANO MORCILLO, Encarnación, PAGEL, Kevin. Ion mobility–mass spectrometry of palytoxin-like compounds produced by Ostreopsis cf. ovata. _Analytical and Bioanalytical Chemistry_. 2025. Vol. 417, núm. 29, pàgs. 6665-6677. [consulta: 27 de gener de 2026]. ISSN: 1618-2642. [Disponible a: https://hdl.handle.net/2445/225916]