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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/206244
Fear extinction is regulated by the activity of long noncoding RNAs at the synapse
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Long noncoding RNAs (lncRNAs) represent a multidimensional class of regulatory molecules that are involved in many aspects of brain function. Emerging evidence indicates that lncRNAs are localized to the synapse; however, a direct role for their activity in this subcellular compartment in memory formation has yet to be demonstrated. Using lncRNA capture-seq, we identified a specific set of lncRNAs that accumulate in the synaptic compartment within the infralimbic prefrontal cortex of adult male C57/Bl6 mice. Among these was a splice variant related to the stress-associated lncRNA, Gas5. RNA immunoprecipitation followed by mass spectrometry and single-molecule imaging revealed that this Gas5 isoform, in association with the RNA binding proteins G3BP2 and CAPRIN1, regulates the activity-dependent trafficking and clustering of RNA granules. In addition, we found that cell-type-specific, activity-dependent, and synapse-specific knockdown of the Gas5 variant led to impaired fear extinction memory. These findings identify a new mechanism of fear extinction that involves the dynamic interaction between local lncRNA activity and RNA condensates in the synaptic compartment. Synaptic activity controls the extinction of conditioned fear. Here the authors discovered a new way that the brain controls memories of fear: a long noncoding RNA called Gas5 that coordinates the activity of RNA granules in the synaptic compartment.
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LIAU, Wei siang, ZHAO, Qiongyi, BADEMOSI, Adekunle, GORMAL, Rachel s., GONG, Hao, MARSHALL, Paul r., PERIYAKARUPPIAH, Ambika, MADUGALLE, Sachithrani u., ZAJACZKOWSKI, Esmi l., LEIGHTON, Laura j., REN, Haobin, MUSGROVE, Mason, DAVIES, Joshua, RAUCH, Simone, HE, Chuan, DICKINSON, Bryan c., LI, Xiang, WEI, Wei, MEUNIER, Frédéric a., FERNÁNDEZ MOYA, Sandra m., KIEBLER, Michael a., SRINIVASAN, Balakumar, BANERJEE, Sourav, CLARK, Michael, SPITALE, Robert c., BREDY, Timothy w.. Fear extinction is regulated by the activity of long noncoding RNAs at the synapse. _Nature Communications_. 2023. Vol. 14, núm. 1. [consulta: 24 de gener de 2026]. ISSN: 2041-1723. [Disponible a: https://hdl.handle.net/2445/206244]