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Article

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Accepted version

Publication date

2010-11-16

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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/179785

Synthesis and properties of small interfering RNA duplexes carrying 5-ethyluridine residues

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https://doi.org/10.1039/c003221b

Abstract

Oligoribonucleotides carrying 5-ethyluridine units were prepared using solid-phase phosphoramidite chemistry. The introduction of the tert-butyldimethylsilyl group at the 2'-OH position proceeded in good yield and very high 2'-regioselectivity. RNA duplexes carrying 5-ethyluridine either at the sense or the guide strands display RNAi activity comparable to or slightly better than that of unmodified RNA duplexes. Gene suppression experiments using luciferase targets in SH-SY5Y cells show that the ethyl group is generally well accepted at all positions although a small decrease in RNA interference activity is observed when one 5-ethylU residue is incorporated in the 3' overhangs.

Subject

RNA, Oligonucleòtids, Química orgànica

Subject (English)

RNA, Oligonucleotides, Organic chemistry

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Articles publicats en revistes (Química Inorgànica i Orgànica)
Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))

Citation

TERRAZAS MARTÍNEZ, Montserrat and ERITJA I CASADELLÀ, Ramon. Synthesis and properties of small interfering RNA duplexes carrying 5-ethyluridine residues. Molecular Diversity. 2010. Vol. 15, num. 3, pags. 677-686. ISSN 1381-1991. [consulted: 14 of August of 2026]. Available at: https://hdl.handle.net/2445/179785

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