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2022_Cells_NeuronMicroglia_LopesCDF.pdf (21.55 MB)

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2022-02-01

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cc by (c) Portugal, Camila Cabral et al., 2022
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/184165

Neuron-Microglia Contact-Dependent Mechanisms Attenuate Methamphetamine-Induced Microglia Reactivity and Enhance Neuronal Plasticity

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https://doi.org/10.3390/cells11030355

Abstract

Exposure to methamphetamine (Meth) has been classically associated with damage to neuronal terminals. However, it is now becoming clear that addiction may also result from the interplay between glial cells and neurons. Recently, we demonstrated that binge Meth administration promotes microgliosis and microglia pro-inflammation via astrocytic glutamate release in a TNF/IP(3)R2-Ca2+-dependent manner. Here, we investigated the contribution of neuronal cells to this process. As the crosstalk between microglia and neurons may occur by contact-dependent and/or contact-independent mechanisms, we developed co-cultures of primary neurons and microglia in microfluidic devices to investigate how their interaction affects Meth-induced microglia activation. Our results show that neurons exposed to Meth do not activate microglia in a cell-autonomous way but require astrocyte mediation. Importantly, we found that neurons can partially prevent Meth-induced microglia activation via astrocytes, which seems to be achieved by increasing arginase 1 expression and strengthening the CD200/CD200r pathway. We also observed an increase in synaptic individual area, as determined by co-localization of pre- and post-synaptic markers. The present study provides evidence that contact-dependent mechanisms between neurons and microglia can attenuate pro-inflammatory events such as Meth-induced microglia activation.

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Estimulants, Neuròglia

Subject (English)

Stimulants, Neuroglia

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Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))

Citation

PORTUGAL, Camila Cabral, et al. Neuron-Microglia Contact-Dependent Mechanisms Attenuate Methamphetamine-Induced Microglia Reactivity and Enhance Neuronal Plasticity. Cells. 2022. Vol.  vol 11, num. num 3. ISSN 2073-4409. [consulted: 10 of June of 2026]. Available at: https://hdl.handle.net/2445/184165

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