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2008-09-08

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cc-by (c) Llobet Berenguer, Artur, 1972- et al., 2008
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/190502

The mouth of a dense-core vesicle opens and closes in a concerted action regulated by calcium and amphiphysin

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https://doi.org/10.1083/jcb.200807034

Abstract

Secretion of hormones and peptides by neuroendocrine cells occurs through fast and slow modes of vesicle fusion but the mechanics of these processes are not understood. We used interference reflection microscopy to monitor deformations of the membrane surface and found that both modes of fusion involve the tightly coupled dilation and constriction of the vesicle. The rate of opening is calcium dependent and occurs rapidly at concentrations <5 muM [corrected] The fast mode of fusion is blocked selectively by a truncation mutant of amphiphysin. Vesicles do not collapse when fusion is triggered by strontium, rather they remain locked open and membrane scission is blocked. In contrast, constriction of the vesicle opening continues when endocytosis is blocked by inhibiting the function of dynamin. Thus, fast and slow modes of fusion involve similar membrane deformations and vesicle closure can be uncoupled from membrane scission. Regulation of these processes by calcium and amphiphysin may provide a mechanism for controlling the release of vesicle contents.

Subject

Calci, Membranes cel·lulars, Animals

Subject (English)

Calcium, Cell membranes, Animals

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Articles publicats en revistes (Patologia i Terapèutica Experimental)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

Citation

LLOBET BERENGUER, Artur, WU, Minnie and LAGNADO, Leon. The mouth of a dense-core vesicle opens and closes in a concerted action regulated by calcium and amphiphysin. Journal of Cell Biology. 2008. Vol. 182, num. 5, pags. 1017-1028. ISSN 0021-9525. [consulted: 15 of August of 2026]. Available at: https://hdl.handle.net/2445/190502

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