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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/112225
Single-cell transcriptome conservation in cryopreserved cells and tissues
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A variety of single-cell RNA preparation procedures have been described. So far, protocols require fresh material, which hinders complex study designs. We describe a sample preservation method that maintains transcripts in viable single cells, allowing one to disconnect time and place of sampling from subsequent processing steps. We sequence single-cell transcriptomes from >1000 fresh and cryopreserved cells using 3'-end and full-length RNA preparation methods. Our results confirm that the conservation process did not alter transcriptional profiles. This substantially broadens the scope of applications in single-cell transcriptomics and could lead to a paradigm shift in future study designs.
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GUILLAUMET ADKINS, Amy, et al. Single-cell transcriptome conservation in cryopreserved cells and tissues. Genome Biology. 2017. Vol. 18, num. 45. ISSN 1474-7596. [consulted: 28 of August of 2026]. Available at: https://hdl.handle.net/2445/112225