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2016-03

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cc-by-nc-nd (c) Moran, Sebastian et al., 2016
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/114129

Validation of a DNA methylation microarray for 850,000 CpG sites of the human genome enriched in enhancer sequences

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https://doi.org/10.2217/epi.15.114

Abstract

DNA methylation is the best known epigenetic mark. Cancer and other pathologies show an altered DNA methylome. However, delivering complete DNA methylation maps is compromised by the price and labor-intensive interpretation of single nucleotide methods. MATERIAL & METHODS: Following the success of the HumanMethylation450 BeadChip (Infinium) methylation microarray (450K), we report the technical and biological validation of the newly developed MethylationEPIC BeadChip (Infinium) microarray that covers over 850,000 CpG methylation sites (850K). The 850K microarray contains >90% of the 450K sites, but adds 333,265 CpGs located in enhancer regions identified by the ENCODE and FANTOM5 projects. RESULTS & CONCLUSION: The 850K array demonstrates high reproducibility at the 450K CpG sites, is consistent among technical replicates, is reliable in the matched study of fresh frozen versus formalin-fixed paraffin-embeded samples and is also useful for 5-hydroxymethylcytosine. These results highlight the value of the MethylationEPIC BeadChip as a useful tool for the analysis of the DNA methylation profile of the human genome.

Subject

Metilació, ADN, Epigènesi, Microxips d'ADN, Genoma humà

Subject (English)

Methylation, DNA, Epigenesis, DNA microarrays, Human genome

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Articles publicats en revistes (Ciències Fisiològiques)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
Publicacions de projectes de recerca finançats per la UE

Citation

MORAN, Sebastian, ARRIBAS, Carles and ESTELLER, Manel. Validation of a DNA methylation microarray for 850,000 CpG sites of the human genome enriched in enhancer sequences. Epigenomics. 2016. Vol. 8, num. 3, pags. 389-399. ISSN 1750-1911. [consulted: 17 of August of 2026]. Available at: https://hdl.handle.net/2445/114129

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