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2021-01-07

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cc-by (c) Planinc, Ivo et al., 2021
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/186967

Comprehensive assessment of myocardial remodeling in ischemic heart disease by synchrotron propagation based X-ray phase contrast imaging.

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https://doi.org/10.1038/s41598-021-93054-6

Abstract

Cardiovascular research is in an ongoing quest for a superior imaging method to integrate grossanatomical information with microanatomy, combined with quantifable parameters of cardiac structure. In recent years, synchrotron radiation-based X-ray Phase Contrast Imaging (X-PCI) has been extensively used to characterize soft tissue in detail. The objective was to use X-PCI to comprehensively quantify ischemic remodeling of diferent myocardial structures, from cell to organ level, in a rat model of myocardial infarction. Myocardial infarction-induced remodeling was recreated in a well-established rodent model. Ex vivo rodent hearts were imaged by propagation based X-PCI using two confgurations resulting in 5.8 µm and 0.65 µm efective pixel size images. The acquired datasets were used for a comprehensive assessment of macrostructural changes including the whole heart and vascular tree morphology, and quantifcation of left ventricular myocardial thickness, mass, volume, and organization. On the meso-scale, tissue characteristics were explored and compared with histopathological methods, while microstructural changes were quantifed by segmentation of cardiomyocytes and calculation of cross-sectional areas. Propagation based X-PCI provides detailed visualization and quantifcation of morphological changes on whole organ, tissue, vascular as well as individual cellular level of the ex vivo heart, with a single, non-destructive 3D imaging modality.

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Diagnòstic per la imatge, Raigs X, Animals

Subject (English)

Diagnostic imaging, X-rays, Animals

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Articles publicats en revistes (Medicina)
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)

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PLANINC, Ivo, et al. Comprehensive assessment of myocardial remodeling in ischemic heart disease by synchrotron propagation based X-ray phase contrast imaging. Scientific Reports. 2021. Vol. 11, num. 14020. ISSN 2045-2322. [consulted: 10 of August of 2026]. Available at: https://hdl.handle.net/2445/186967

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