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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/116589
Improving virus production through quasispecies genomic selection and molecular breedings
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Virus production still is a challenging issue in antigen manufacture, particularly with slow-growing viruses. Deep-sequencing of genomic regions indicative of efficient replication may be used to identify high-fitness minority individuals suppressed by the ensemble of mutants in a virus quasispecies. Molecular breeding of quasispecies containing colonizer individuals, under regimes allowing more than one replicative cycle, is a strategy to select the fittest competitors among the colonizers. A slow-growing cell culture-adapted hepatitis A virus strain was employed as a model for this strategy. Using genomic selection in two regions predictive of efficient translation, the internal ribosome entry site and the VP1-coding region, high-fitness minority colonizer individuals were identified in a population adapted to conditions of artificially-induced cellular transcription shut-off. Molecular breeding of this population with a second one, also adapted to transcription shut-off and showing an overall colonizer phenotype, allowed the selection of a fast-growing population of great biotechnological potential.
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PÉREZ-RODRÍGUEZ, Francisco Javier, et al. Improving virus production through quasispecies genomic selection and molecular breedings. Scientific Reports. 2016. Vol. 6, num. 35962. ISSN 2045-2322. [consulted: 9 of June of 2026]. Available at: https://hdl.handle.net/2445/116589