Amb motiu del tancament d'estiu, la validació de documents es reprendrà a partir del 28 d'agost de 2026. Disculpeu les molèsties.
Con motivo del cierre de verano, la validación de documentos se reanudará a partir del 28 de agosto de 2026. Disculpad las molestias
Due to the summer closure, document validation will resume starting August 28, 2026. We apologize for any inconvenience.

Fitxers

664989.pdf (1.58 MB)

Tipus de document

Article

Versió

Versió publicada

Data de publicació

2016-11-03

Llicència de publicació

cc-by (c) Pérez et al., 2016
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/116589

Improving virus production through quasispecies genomic selection and molecular breedings

Títol de la revista

Autors

Director/Tutor

ISSN de la revista

Títol del volum

Recurs relacionat

https://doi.org/10.1038/srep35962

Resum

Virus production still is a challenging issue in antigen manufacture, particularly with slow-growing viruses. Deep-sequencing of genomic regions indicative of efficient replication may be used to identify high-fitness minority individuals suppressed by the ensemble of mutants in a virus quasispecies. Molecular breeding of quasispecies containing colonizer individuals, under regimes allowing more than one replicative cycle, is a strategy to select the fittest competitors among the colonizers. A slow-growing cell culture-adapted hepatitis A virus strain was employed as a model for this strategy. Using genomic selection in two regions predictive of efficient translation, the internal ribosome entry site and the VP1-coding region, high-fitness minority colonizer individuals were identified in a population adapted to conditions of artificially-induced cellular transcription shut-off. Molecular breeding of this population with a second one, also adapted to transcription shut-off and showing an overall colonizer phenotype, allowed the selection of a fast-growing population of great biotechnological potential.

Matèries

Virologia, Espècies (Biologia)

Matèries (anglès)

Virology, Species

Citació

Col·leccions

Articles publicats en revistes (Genètica, Microbiologia i Estadística)
Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)

Citació

PÉREZ-RODRÍGUEZ, Francisco Javier, et al. Improving virus production through quasispecies genomic selection and molecular breedings. Scientific Reports. 2016. Vol. 6, num. 35962. ISSN 2045-2322. [consulted: 14 of August of 2026]. Available at: https://hdl.handle.net/2445/116589

Exportar metadades

JSON - METS

Compartir registre