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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/189942
Identification and Quantification of Urinary Microbial Phenolic Metabolites by HPLC-ESI-LTQ-Orbitrap-HRMS and Their Relationship with Dietary Polyphenols in Adolescents
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This study aimed to develop and validate a liquid chromatography/electrospray ionization-linear ion trap quadrupole-Orbitrap-high-resolution mass spectrometry (HPLC/ESI-LTQ-Orbitrap-HRMS) method to identify and quantify urinary microbial phenolic metabolites (MPM), as well as to explore the relationship between MPM and dietary (poly)phenols in Spanish adolescents. A total of 601 spot urine samples of adolescents aged 12.02 ± 0.41 years were analyzed. The quantitative method was validated for linearity, limit of detection, limit of quantification, recovery, intra-and inter-day accuracy and precision, as well as postpreparative stability according to the criteria established by the Association of Official Agricultural Chemists International. A total of 17 aglycones and 37 phase II MPM were identified and quantified in 601 spot urine samples. Phenolic acids were the most abundant urinary MPM, whereas stilbenes, hydroxytyrosol, and enterodiol were the least abundant. Urinary hydroxycoumarin acids (urolithins) were positively correlated with flavonoid and total (poly)phenol intake. An HPLC-ESI-LTQ-Orbitrap-HRMS method was developed and fully validated to quantify MPM. The new method was performed accurately and is suitable for MPM quantification in large epidemiological studies. Urinary lignans and urolithins are proposed as potential biomarkers of grain and nut intake in an adolescent population.
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LAVERIANO-SANTOS, Emily p., MARHUENDA-MUÑOZ, María, VALLVERDÚ I QUERALT, Anna, MARTÍNEZ HUÉLAMO, Miriam, TRESSERRA I RIMBAU, Anna, MILIARAKIS, Eleftherios, ARANCIBIA RIVEROS, Camila, JÁUREGUI PALLARÉS, Olga, RUÍZ LEÓN, Ana maría, CASTRO-BARQUERO, Sara, ESTRUCH, Ramon, BODEGA, Patricia, MIGUEL, Mercedes de, COS GANDOY, Amaya de, MARTÍNEZ GOMEZ, Jesús, SANTOS-BENEIT, Gloria, FERNÁNDEZ ALVIRA, Juan miguel, FERNÁNDEZ JIMÉNEZ, Rodrigo, LAMUELA RAVENTÓS, Rosa ma.. Identification and Quantification of Urinary Microbial Phenolic Metabolites by HPLC-ESI-LTQ-Orbitrap-HRMS and Their Relationship with Dietary Polyphenols in Adolescents. _Antioxidants_. 2022. Vol. 11, núm. 6, pàgs. 1167. [consulta: 24 de gener de 2026]. ISSN: 2076-3921. [Disponible a: https://hdl.handle.net/2445/189942]