Document type
ArticleVersion
Accepted versionPublication date
Publication license
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/178889
Ethylcellulose nanoparticles as a new "in vitro" transfection tool for antisense oligonucleotide delivery
Journal Title
Director/Tutor
Journal ISSN
Volume Title
Related resource
Abstract
Oil-in-water nano-emulsions have been obtained in the HEPES 20 mM buffer solution / [Alkylamidoammonium:Kolliphor EL = 1:1] / [6 wt% ethylcellulose in ethyl acetate] system over a wide oil-to-surfactant range and above 35 wt% aqueous component at 25 °C. The nano-emulsion with an oil-to-surfactant ratio of 70/30 and 95 wt% aqueous component was used for nanoparticles preparation. These nanoparticles (mean diameter around 90 nm and zeta potential of +22 mV) were non-toxic to HeLa cells up to a concentration of 3 mM of cationic species. Successful complexation with an antisense phosphorothioate oligonucleotide targeting Renilla luciferase mRNA was achieved at cationic/anionic charge ratios above 16, as confirmed by zeta potential measurements and an electrophoretic mobility shift assay, provided that no Fetal Bovine Serum is present in the cell culture medium. Importantly, Renilla luciferase gene inhibition shows an optimum efficiency (40%) for the cationic/anionic ratio 28, which makes these complexes promising for "in vitro" cell transfection.
Subject
Subject (English)
Citation
Citation
LEITNER, Stefanie, et al. Ethylcellulose nanoparticles as a new "in vitro" transfection tool for antisense oligonucleotide delivery. Carbohydrate Polymers. 2020. Vol. 229. ISSN 0144-8617. [consulted: 15 of August of 2026]. Available at: https://hdl.handle.net/2445/178889