Files
Document type
ArticleVersion
Published versionPublication date
Publication license
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/154360
Self-association of short DNA loops through minor groove C:G:G:C tetrads
Journal Title
Director/Tutor
Journal ISSN
Volume Title
Related resource
Abstract
In addition to the better known guanine-quadruplex, four-stranded nucleic acid structures can be formed by tetrads resulting from the association of Watson-Crick base pairs. When such association occurs through the minor groove side of the base pairs, the resulting structure presents distinctive features, clearly different from quadruplex structures containing planar G-tetrads. Although we have found this unusual DNA motif in a number of cyclic oligonucleotides, this is the first time that this DNA motif is found in linear oligonucleotides in solution, demonstrating that cyclization is not required to stabilize minor groove tetrads in solution. In this article, we have determined the solution structure of two linear octamers of sequence d(TGCTTCGT) and d(TCGTTGCT), and their cyclic analogue d, utilizing 2D NMR spectroscopy and restrained molecular dynamics. These three molecules self-associate forming symmetric dimers stabilized by a novel kind of minor groove C:G:G:C tetrad, in which the pattern of hydrogen bonds differs from previously reported ones. We hypothesize that these quadruplex structures can be formed by many different DNA sequences, but its observation in linear oligonucleotides is usually hampered by competing Watson-Crick duplexes.
Subject (English)
Citation
Citation
VILADOMS CLAVEROL, Júlia, et al. Self-association of short DNA loops through minor groove C:G:G:C tetrads. Nucleic Acids Research. 2009. Vol. 37, num. 10, pags. 3264 -3275. ISSN 0305-1048. [consulted: 12 of August of 2026]. Available at: https://hdl.handle.net/2445/154360