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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/227048
Characterization of myogenesis in European sea bass (Dicentrarchus labrax) using primary white muscle cell cultures
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This study presents the first characterization of a primary cell culture from white skeletal muscleof European sea bass (Dicentrarchus labrax). Using immunofluorescence and gene expressionanalyses over 12 days, cell activation, proliferation, differentiation, fusion, and maturation phaseswere described. During culture development, myogenic regulatory factors (myf5, myod1, myod2,myog, mrf4) were sequentially expressed. Proliferation peaked at days 4–6, with high Pcna andMyod immunodetection and gene expression of pax7, c-met, and pcna. Early downregulationof cell cycle regulators, cdkn1a and cdkn1cb, and mstnb may have contributed to proliferation,while cdkn1bb progressively increased, likely to promote differentiation. The Gh/Igf axis showeddifferential regulation, igf-1 decreasing early and igf-2, igf-1ra, igf-1rb, and igfbp-1a gradually rising.Differentiation, myotube formation, and maturation were marked by higher Myhc staining, sarcomeredevelopment, and upregulation of cdh15, cav3, mef2, mymk, mymx, myhcb, and wnt4. Anabolic (akt2,mtor, eif4ebp1) and proteolytic-related genes (foxo1a, murf1, mafbx, capn1, capn3b, atg12, map1lc3b)increased in later stages. Comparison with other vertebrates revealed both conserved and speciesspecificregulatory mechanisms of myogenesis. These findings provide a comprehensive molecularframework of skeletal muscle development in European sea bass and establish a valuable in vitromodel for studying fish muscle biology and potential aquaculture and biotechnology applications.
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GARCÍA-PÉREZ, Isabel, et al. Characterization of myogenesis in European sea bass (Dicentrarchus labrax) using primary white muscle cell cultures. Scientific Reports. 2025. Vol. 15, num. 45778. ISSN 2045-2322. [consulted: 6 of June of 2026]. Available at: https://hdl.handle.net/2445/227048