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2024-12-23

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cc-by (c) Silva klug, Ana C et al., 2024
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/218881

Using anal cytology and HPV DNA and E6/E7 mRNA detection to optimize high-resolution anoscopy referrals in men who have sex with men living with HIV

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Background This study was conducted to evaluate screening procedures for anal high-grade squamous intraepithelial lesions (HSILs) with anal liquid-based cytology (aLBC) and biomarkers to identify candidates for high-resolution anoscopy (HRA).Methods This cross-sectional study included men who have sex with men with HIV. Participants underwent HRA, aLBC, and biomarker testing. Three screening procedures were compared with aLBC: biomarker alone, cytology and biomarker in all, and cytology and reflex biomarkers (biomarkers applied if aLBC results were atypical squamous cells of undetermined significance or low-grade squamous intraepithelial lesion). Biomarkers included Linear Array (LA), LA for 14 high-risk human papillomavirus (LA 14 HR-HPV) genotypes, LA HPV-16, Hybrid Capture 2 (HC2), E6/E7 mRNA, and E6/E7 mRNA HPV-16.Results Of 354 participants, 179 (50.6%) had atypical squamous cells of undetermined significance or worse, requiring HRA (sensitivity, 80%; specificity, 57.3%; area under the curve, 0.687; reference, biopsy-proven HSIL). Cytology and reflex biomarkers per E6/E7 mRNA, LA 14 HR-HPV, and HC2 and the biomarker-alone procedure with HC2 showed comparable accuracy (sensitivities: 71.6%, 78.8%, 73.1%, 75.7%; specificities: 73.5%, 67.9%, 76.1%, 65.5%; areas under the curve: 0.726, 0.734, 0.746, 0.706) with fewer HRA referrals (number needed to diagnose: 2.2, 2.1, 2, 2.4).Conclusions Our findings suggest that E6/E7 mRNA, LA 14 HR-HPV, and HC2 in the cytology and reflex biomarkers procedure, as well as HC2 in the biomarker-alone procedure, can improve anal HSIL screening effectiveness.

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Malalties de l'anus, Papil·lomavirus, VIH (Virus), Citologia

Matèries (anglès)

Diseases of the anus, Papillomaviruses, HIV (Viruses), Cytology

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Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
Articles publicats en revistes (ISGlobal)

Citació

SILVA KLUG, Ana c, PAYTUBI CASABONA, Sònia, TORRES, Montserrat, TRENTI, Loris, BAIXERAS, Nuria, SANCHEZ LLAMAS, Monica, PAVÓN, Miquel ángel, SANJOSE, Silvia de, CATALA, Isabel, VIDAL, August, POLJAK, Mario, ALEMANY, Laia, PODZAMCZER PALTER, Daniel, VIDELA, Sebastià, SAUMOY, Maria. Using anal cytology and HPV DNA and E6/E7 mRNA detection to optimize high-resolution anoscopy referrals in men who have sex with men living with HIV. _Open Forum Infectious Diseases_. 2024. Vol. 12, núm. 1. [consulta: 24 de novembre de 2025]. ISSN: 2328-8957. [Disponible a: https://hdl.handle.net/2445/218881]

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